电针通过改善视网膜线粒体功能减缓实验性近视进展：一项基于单细胞RNA测序的研究

Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing.

作者信息

Hao Jiawen, Xie Yunxiao, Wei Huixia, Yang Zhaohui, Zhang Ruixue, Ma Zhongyu, Zhang Miao, Du Xiaoshi, Yin Xuewei, Liu Jinpeng, Bao Bo, Bi Hongsheng, Guo Dadong

机构信息

Shandong University of Traditional Chinese Medicine, Jinan, 250002, China.

Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Academy of Eye Disease Prevention and Therapy, Shandong Provincial Clinical Research Center of Ophthalmology and Children Visual Impairment Prevention and Control, Shandong Engineering Technology Research Center of Visual Intelligence, Shandong Academy of Health and Myopia Prevention and Control of Children and Adolescents, Jinan, 250002, China.

出版信息

Adv Biol (Weinh). 2024 Dec;8(12):e2400269. doi: 10.1002/adbi.202400269. Epub 2024 Oct 15.

DOI:10.1002/adbi.202400269

PMID:39404059

Abstract

This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is used to assess changes in individual cellular gene levels in the retina of LIM- and EA-treated guinea pigs. In addition, the role of EA in slowing myopia progression by improving retinal mitochondrial function is further investigated. scRNA-seq identified ten cell clusters in the retina of LIM and EA guinea pigs and mitochondrial respiratory chain-related genes in Cones and Muller-glia cells-Cytochrome oxidase subunit III (COX3), NADH dehydrogenase subunit 4 (ND4), and NADH dehydrogenase subunit 2 (ND2) are closely related to lens-induced myopia. A comprehensive atlas in the retina of LIM and EA guinea pigs at a single-cell level is established, and the positive role of EA in improving retinal mitochondrial function to slow the experimental myopia progression in guinea pigs is revealed.

摘要

本研究旨在通过单细胞RNA测序（scRNA-seq）建立晶状体诱导性近视（LIM）和电针（EA）干预下视网膜细胞的完整图谱，并探讨电针改善豚鼠实验性近视进展的潜在机制。scRNA-seq用于评估LIM和EA处理的豚鼠视网膜中单个细胞基因水平的变化。此外，进一步研究电针通过改善视网膜线粒体功能在减缓近视进展中的作用。scRNA-seq在LIM和EA豚鼠的视网膜中鉴定出十个细胞簇，并且视锥细胞和穆勒胶质细胞中的线粒体呼吸链相关基因——细胞色素氧化酶亚基III（COX3）、烟酰胺腺嘌呤二核苷酸脱氢酶亚基4（ND4）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基2（ND2）与晶状体诱导性近视密切相关。建立了LIM和EA豚鼠视网膜在单细胞水平的综合图谱，并揭示了电针在改善视网膜线粒体功能以减缓豚鼠实验性近视进展方面的积极作用。