Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, Poland.
Cells. 2024 Sep 24;13(19):1603. doi: 10.3390/cells13191603.
Adipose-derived mesenchymal stem cells (ADMSCs) are progenitor cells that shape the tissue's biological properties. To examine the adipocytes differentiated from the ADMSCs of lean and obese individuals with/without a metabolic syndrome (MetSx) cytokine secretory profile, as to date, little is known on this topic. Interleukin, chemokine and growth factor levels in the culture medium were determined using the Human Cytokine kit. : We observed a characteristic secretory fingerprint displayed by the cells from the MetSx group and identified a set of putative markers (IL-1β, IL-6, IL-7, IL-10, IL-12, IL-13, VEGF, FGF, GM-CSF, TNF-α, IFN-γ) of the condition. Surprisingly, the concentrations of most of the molecules (except for IL-6, IFN-γ, IP-10, VEGF) decreased when compared with the cells from the lean group. We postulate that the difference stemmed from the fact that in vivo cytokines were mostly secreted by the activated monocytes/macrophages and not adipocytes per se. This may also suggest that the aforementioned upregulated cytokines (IL-6, IFN-γ, IP-10, VEGF) might have been the ones that attracted monocytes and triggered the vicious cycle of tissue inflammation. : Our study indicated that the adipocytes newly derived from the ADMSCs of obese patients with metabolic syndrome displayed a secretory fingerprint that may be characteristic to the early stages of the condition.
脂肪间充质干细胞（ADMSCs）是一种祖细胞，可塑造组织的生物学特性。为了研究瘦人和肥胖个体（伴或不伴代谢综合征（MetSx））的 ADMSCs 分化而来的脂肪细胞的细胞因子分泌特征，迄今为止，这方面的研究还很少。使用人细胞因子试剂盒测定培养基中的白细胞介素、趋化因子和生长因子水平。我们观察到来自 MetSx 组的细胞显示出特征性的分泌特征，并确定了一组可能的标志物（IL-1β、IL-6、IL-7、IL-10、IL-12、IL-13、VEGF、FGF、GM-CSF、TNF-α、IFN-γ）。令人惊讶的是，与来自瘦组的细胞相比，大多数分子（除了 IL-6、IFN-γ、IP-10、VEGF）的浓度降低。我们推测，这种差异源于这样一个事实，即在体内细胞因子主要由激活的单核细胞/巨噬细胞而不是脂肪细胞本身分泌。这也可能表明，上述上调的细胞因子（IL-6、IFN-γ、IP-10、VEGF）可能是吸引单核细胞并引发组织炎症恶性循环的细胞因子。我们的研究表明，来自肥胖伴代谢综合征患者的 ADMSCs 新分化的脂肪细胞显示出一种可能是该疾病早期特征的分泌特征。
