Department of Physiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.
Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Waszyngtona 13 Street, 15-269 Bialystok, Poland.
Cells. 2022 Apr 23;11(9):1435. doi: 10.3390/cells11091435.
Adipose tissue is an abundant source of mesenchymal stem cells (ADMSCs). Evidence has suggested that depot-specific ADMSCs (obtained from subcutaneous or visceral adipose tissue-subADMSCs or visADMSCs, respectively) account for differential responses of each depot to metabolic challenges. However, little is known about the phenotype and changes in metabolism of the adipocytes derived from ADMSCs of obese individuals. Therefore, we investigated the phenotypic and metabolic characteristics, particularly the lipid profile, of fully differentiated adipocytes derived from ADMSCs of lean and obese (with/without metabolic syndrome) postmenopausal women. We observed a depot-specific pattern, with more pronounced changes present in the adipocytes obtained from subADMSCs. Namely, chronic oversupply of fatty acids (present in morbid obesity) triggered an increase in CD36/SR-B2 and FATP4 protein content (total and cell surface), which translated to an increased LCFA influx (H-palmitate uptake). This was associated with the accumulation of TAG and DAG in these cells. Furthermore, we observed that the adipocytes of visADMSCs origin were larger and showed smaller granularity than their counterparts of subADMSCs descent. Although ADMSCs were cultured in vitro, in a fatty acids-deprived environment, obesity significantly influenced the functionality of the progenitor adipocytes, suggesting the existence of a memory effect.
脂肪组织是间充质干细胞（ADMSCs）的丰富来源。有证据表明，特定部位的 ADMSCs（分别从皮下或内脏脂肪组织中获得的 ADMSCs-亚 ADMSCs 或内脏 ADMSCs）解释了每个部位对代谢挑战的不同反应。然而，对于肥胖个体的 ADMSCs 来源的脂肪细胞的表型和代谢变化知之甚少。因此，我们研究了源自瘦和肥胖（有/无代谢综合征）绝经后妇女的 ADMSCs 的完全分化脂肪细胞的表型和代谢特征，特别是脂谱。我们观察到一种特定部位的模式，亚 ADMSCs 获得的脂肪细胞表现出更明显的变化。即，脂肪酸的慢性过度供应（存在于病态肥胖中）引发 CD36/SR-B2 和 FATP4 蛋白含量（总蛋白和细胞表面）的增加，这转化为 LCFA 流入（H-软脂酸摄取）的增加。这与这些细胞中 TAG 和 DAG 的积累有关。此外，我们观察到，源自内脏 ADMSCs 的脂肪细胞比源自亚 ADMSCs 的脂肪细胞更大，颗粒更小。尽管 ADMSCs 在体外培养，处于脂肪酸缺乏的环境中，但肥胖显著影响了祖细胞脂肪细胞的功能，这表明存在记忆效应。
