Shaker Alaa A, Samir Ahmed, Zaher Hala M, Abdel-Moein Khaled A
Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Vector Borne Zoonotic Dis. 2025 Feb;25(2):118-124. doi: 10.1089/vbz.2024.0082. Epub 2024 Oct 15.
Researchers paid more attention to nosocomial in veterinary hospitals worldwide; however, the research scope toward community-acquired infections among animals is largely ignored. Therefore, the current study aimed to investigate the role of diseased dogs and cats suffering from respiratory illness in transmission of community-acquired infection and its public health threat. Oral swabs were collected from 154 pet animals with respiratory signs, including 80 cats and 74 dogs (outpatient visits). The obtained swabs were cultured on CHROMagar™ MH Orientation media for isolation of , and identification of suspected isolates was conducted via Gram staining, conventional biochemical tests, and molecular detection of the gene. Antimicrobial susceptibility testing of isolates was carried out using the disc diffusion method. Overall, 10 (6.5%) out of 154 diseased pet animals were positive for , where 6 (8.1%) and 4 (5%) dogs and cats were positive, respectively. Multidrug-resistant (MDR) was found in 3.9% of the examined animals. The phylogenetic tree analysis revealed that the obtained sequences from dogs and cats were closely related to human and animal sequences. The occurrence of MDR among dogs and cats suffering from respiratory illness highlights the potential role of pet animals in the dissemination of MDR in the community.
全球范围内，研究人员对兽医医院的医院感染更为关注；然而，动物社区获得性感染的研究范围在很大程度上被忽视了。因此，本研究旨在调查患有呼吸道疾病的病犬和病猫在社区获得性感染传播中的作用及其对公共卫生的威胁。从154只出现呼吸道症状的宠物中采集口腔拭子，其中包括80只猫和74只狗（门诊病例）。将获得的拭子接种在CHROMagar™ MH定向培养基上以分离[未明确提及的某种细菌]，并通过革兰氏染色、常规生化试验以及[未明确提及的某种基因]的分子检测对疑似分离株进行鉴定。对分离株进行药敏试验采用纸片扩散法。总体而言，154只患病宠物中有10只（占6.5%）[未明确提及的某种细菌]呈阳性，其中狗和猫分别有6只（占8.1%）和4只（占5%）呈阳性。在3.9%的受检动物中发现了多重耐药[未明确提及的某种细菌]。系统发育树分析表明，从狗和猫获得的序列与人类和动物序列密切相关。患有呼吸道疾病的狗和猫中多重耐药[未明确提及的某种细菌]的出现凸显了宠物在社区中传播多重耐药[未明确提及的某种细菌]方面的潜在作用。
