β-石竹烯对精子冷冻保存的影响。
Effect of β-caryophyllene on Sperm Cryopreservation.
作者信息
Moura Emanuela Ribeiro, Branco Marlon de Araújo Castelo, Branco Yndyra Nayan Teixeira Carvalho Castelo, Souza José Adalmir Torres de, Nascimento Isolda Márcia Rocha do, Sousa Marcimar Silva, Oliveira Nathyelle Maria Sousa de, Melo Wanderson Gabriel Gomes de, Bezerra Dayseanny de Oliveira, Sousa Marina Rebeca Soares Carneiro de, Kunkel Daniela, Carvalho Camila Ernanda Sousa de, Muratori Maria Christina Sanches, Sousa Júnior Antônio de, Costa Amilton Paulo Raposo
机构信息
Federal University of Piaui Post-Graduate Program in Tropical Zootechnics Teresina Piauí Brazil Post-Graduate Program in Tropical Zootechnics/Federal University of Piaui, Teresina, Piauí, Brazil.
University Mauricio de Nassau Teresina Piauí Brazil University Mauricio de Nassau, Teresina, Piauí, Brazil.
出版信息
JBRA Assist Reprod. 2024 Dec 3;28(4):670-677. doi: 10.5935/1518-0557.20240060.
OBJECTIVE
This study aimed to assess the impact of β-caryophyllene (BC) supplementation in the extender on the post-cryopreservation quality of semen from Dorper rams.
METHODS
Six Dorper rams were utilized for semen collection over 16 weeks, with BC concentrations determined via the MTT test. Animals were divided into a control group and three treatment groups receiving BC at concentrations of 1.0mM, 2.0mM, and 3.0mM in the Trisegg yolk diluent. Semen was cryopreserved and stored in liquid nitrogen for at least 15 days. After thawing, in vitro assessments including CASA, acrosomal integrity, plasma membrane integrity, mitochondrial membrane potential, and thermo-resistance tests were conducted. Additionally, the TBARS assay was performed to evaluate oxidative stress.
RESULTS
While BC supplementation did not significantly affect sperm motility, it notably improved mitochondrial potential and mitigated oxidative stress in cryopreserved ram semen.
CONCLUSIONS
Incorporating β-caryophyllene into the extender exhibited beneficial effects on the quality of Dorper ram semen post-cryopreservation, enhancing mitochondrial functionality and reducing oxidative stress.
目的
本研究旨在评估在稀释液中添加β-石竹烯（BC）对杜泊种公羊冷冻精液保存后质量的影响。
方法
在16周内使用6只杜泊种公羊采集精液，通过MTT试验测定BC浓度。将动物分为对照组和三个处理组，在Tris-蛋黄稀释液中分别添加浓度为1.0mM、2.0mM和3.0mM的BC。精液冷冻保存并在液氮中储存至少15天。解冻后，进行包括计算机辅助精子分析（CASA）、顶体完整性、质膜完整性、线粒体膜电位和耐热性测试在内的体外评估。此外，进行硫代巴比妥酸反应物（TBARS）测定以评估氧化应激。
结果
虽然添加BC对精子活力没有显著影响，但它显著改善了冷冻保存的公羊精液的线粒体电位并减轻了氧化应激。
结论
在稀释液中加入β-石竹烯对杜泊种公羊冷冻精液保存后的质量具有有益影响，可增强线粒体功能并降低氧化应激。
Zotero 插件