Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science & Technology, Xinjiang University, Urumqi, 830046, China; School of Pharmaceutical Sciences and Institute of Materia Medica, Xinjiang University, Urumqi, 830017, China.
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science & Technology, Xinjiang University, Urumqi, 830046, China; School of Pharmaceutical Sciences and Institute of Materia Medica, Xinjiang University, Urumqi, 830017, China.
Talanta. 2025 Jan 1;282:127020. doi: 10.1016/j.talanta.2024.127020. Epub 2024 Oct 11.
Alzheimer's disease (AD) is a chronic neurodegenerative disease that threatens to the health of global elderly population. Acetylcholinesterase (AChE) inhibitors are an effective therapeutic agent for AD, and screening of these substances is important for AD treatment. In this work, a Pd-Pt MXene nanoenzyme was successfully synthesized by using the in-situ reduction technique. A colorimetric method for sensitive AChE inhibitor detection was designed based on enzymatic cascade reaction between Pd-Pt MXene and AChE. Briefly, The Pd-Pt MXene material exhibited excellent peroxidase (POD)-like activity due to its bimetallic composition, effectively catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzidine (TMB) to generate blue oxidized TMB (oxTMB). Under the presence of AChE and acetylthiocholine chloride (ATCh), the POD-like activity of Pd-Pt MXene was significantly inhibited. The activity of this nanoenzyme could be restored after the addition of AChE inhibitors. Using donepezil as an example, colorimetric detection was conducted within a linear range of 0.1 nmol/L to 10 nmol/L and the lowest detection boundary was only 0.35 nmol/L (S/N = 3). Finally, a paper-based platform was designed and constructed, and it has been successfully employed for AChE inhibitor detection in real samples with the aid of a smartphone. In all, this work paves a new way for designing nanoenzyme-based devices towards medicine determination or screening like AChE inhibitor.
阿尔茨海默病（AD）是一种慢性神经退行性疾病，威胁着全球老年人口的健康。乙酰胆碱酯酶（AChE）抑制剂是治疗 AD 的有效药物，筛选这些物质对 AD 的治疗至关重要。在这项工作中，通过原位还原技术成功合成了 Pd-Pt MXene 纳米酶。基于 Pd-Pt MXene 与 AChE 之间的酶级联反应，设计了一种用于灵敏 AChE 抑制剂检测的比色法。简而言之，由于其双金属组成，Pd-Pt MXene 材料表现出优异的过氧化物酶（POD）样活性，可有效催化无色 3,3,5,5-四甲基联苯胺（TMB）的氧化生成蓝色氧化 TMB（oxTMB）。在存在 AChE 和乙酰硫代胆碱氯化物（ATCh）的情况下，Pd-Pt MXene 的 POD 样活性显著受到抑制。加入 AChE 抑制剂后，这种纳米酶的活性可以恢复。以多奈哌齐为例，比色检测的线性范围为 0.1 nmol/L 至 10 nmol/L，最低检测限仅为 0.35 nmol/L（S/N = 3）。最后，设计并构建了一种基于纸张的平台，并借助智能手机成功用于实际样品中 AChE 抑制剂的检测。总之，这项工作为设计基于纳米酶的用于医学测定或筛选的设备开辟了新途径，如 AChE 抑制剂。
