The Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
Department of Clinical Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.
Talanta. 2025 Jan 1;282:127025. doi: 10.1016/j.talanta.2024.127025. Epub 2024 Oct 9.
Circulating tumor cells (CTCs) are tumor cells that exist in human peripheral blood, which could spread to other tissues or organs via the blood circulation system and develop into metastatic foci, leading to tumor recurrence or metastasis in postoperative patients and thereby increasing the mortality of malignant tumor patients. Evaluation of CTC levels can be used for tumor metastasis prediction, prognosis evaluation, drug exploitation, individualized treatment, liquid biopsy, etc., which exhibit outstanding clinical application prospects. In recent years, accurately capturing and analyzing CTCs has become a research hotspot in the early diagnosis and precise treatment of tumors. This review summarized various enrichment and isolation technologies for evaluating CTCs based on the design principle and discussed the challenges and perspectives in this field.
循环肿瘤细胞（CTCs）是存在于人体外周血中的肿瘤细胞，它们可以通过血液循环系统扩散到其他组织或器官，并发展成转移灶，导致术后患者的肿瘤复发或转移，从而增加恶性肿瘤患者的死亡率。评估 CTC 水平可用于肿瘤转移预测、预后评估、药物开发、个体化治疗、液体活检等，具有突出的临床应用前景。近年来，准确捕获和分析 CTCs 已成为肿瘤早期诊断和精准治疗的研究热点。本文综述了基于设计原理评价 CTCs 的各种富集和分离技术，并讨论了该领域的挑战和展望。
