State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Quality and Health of Tianjin, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Quality and Health of Tianjin, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Food Chem. 2025 Feb 1;464(Pt 1):141570. doi: 10.1016/j.foodchem.2024.141570. Epub 2024 Oct 10.
A capsaicinoids (CPCs) broad spectrum monoclonal antibody with same recognition ability to capsaicin (CPC), dihydrocapsaicin (DCPC), nordihydrocapsaicin (NDCPC), and N-vanillylnonanamide (NV) is prepared. Chitosan (CS) hydrogel is used as the carrier of multicolor quantum dots (QDs) to prepare fluorescence hydrogel beads, CPCs and aflatoxin B (AFB) antibody are coupled with fluorescence hydrogel beads to prepare signal probes. Using AuNPs (or AgNPs) as fluorescence quenching agent to prepare quenching probes followed forming a fluorescence quenching test system. Based on optimal group of signal and quenching probes, a novel, simple, convenient, and ultra-sensitive homogeneous fluorescence immunoassay for the simultaneous detection of CPCs and AFB is constructed. The limit of detection (LOD) of assay for AFB and CPC is 0.00064 μg L and 0.00049 μg L, respectively. This method can realize the simultaneous rapid detection of AFB and CPCs in food, which provides a new strategy for the identification of kitchen waste oil.
一种辣椒素（CPC）广谱单克隆抗体，对辣椒素（CPC）、二氢辣椒素（DCPC）、降二氢辣椒素（NDCPC）和 N-香草基壬酰胺（NV）具有相同的识别能力。壳聚糖（CS）水凝胶用作多色量子点（QDs）的载体，以制备荧光水凝胶珠，将辣椒素（CPC）和黄曲霉毒素 B（AFB）抗体与荧光水凝胶珠偶联，制备信号探针。使用金纳米颗粒（或银纳米颗粒）作为荧光猝灭剂来制备猝灭探针，然后形成荧光猝灭测试系统。基于最佳的信号和猝灭探针组，构建了一种新颖、简单、方便和超灵敏的用于同时检测 CPC 和 AFB 的均相荧光免疫分析方法。该测定法对 AFB 和 CPC 的检测限（LOD）分别为 0.00064μg/L 和 0.00049μg/L。该方法可以实现食品中 AFB 和 CPCs 的同时快速检测，为厨余废油的鉴别提供了新策略。
