基于 GSH 破坏 MnO@QDs 的荧光免疫分析用于谷物中四种真菌毒素的同时超灵敏检测。

A fluorescence immunoassay based on GSH destroying MnO@QDs for the simultaneous ultrasensitive detection of four mycotoxins in cereals.

机构信息

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, Key Laboratory of Food Quality and Health of Tianjin, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.

出版信息

Food Chem. 2023 Sep 15;420:136099. doi: 10.1016/j.foodchem.2023.136099. Epub 2023 Apr 6.

DOI:10.1016/j.foodchem.2023.136099

PMID:37037114

Abstract

A novel fluorescence immunoassay based on MnO nanoflowers loading multicolor quantum dots and glutathione destroying MnO nanoflowers to release quantum dots combined with magnetic separation is developed for rapid, ultra-sensitive, and simultaneous quantitative detection of ochratoxin A, aflatoxin B, fumonisin B, and zearalenone in cereal samples. The test linear range of assay is from 0.001 to 200 μg L. The limit of detection for ochratoxin A, aflatoxin B, fumonisin B, and zearalenone is 0.0001 μg L, 0.0001 μg L, 0.0003 μg L, and 0.0001 μg L, respectively. The simultaneous detection of four mycotoxins can be achieve within 30 min. The test results of four mycotoxins in the incurred corn, rice, and oat samples have been confirmed by ultra-performance liquid chromatography tandem mass spectrometry, the differences between results are considered no significantly different (p > 0.05). This multiplexed test scheme has provided a potential analysis strategy for multiple food risk factors.

摘要

一种基于 MnO 纳米花负载多色量子点和谷胱甘肽破坏 MnO 纳米花释放量子点并结合磁分离的新型荧光免疫分析方法，用于快速、超灵敏、同时定量检测谷物样品中的赭曲霉毒素 A、黄曲霉毒素 B、伏马菌素 B 和玉米赤霉烯酮。该分析方法的检测线性范围为 0.001 至 200μg/L。赭曲霉毒素 A、黄曲霉毒素 B、伏马菌素 B 和玉米赤霉烯酮的检测限分别为 0.0001μg/L、0.0001μg/L、0.0003μg/L 和 0.0001μg/L。四种真菌毒素的同时检测可在 30min 内完成。超高效液相色谱串联质谱法对玉米、大米和燕麦中四种真菌毒素的检测结果进行了验证，结果差异无统计学意义（p＞0.05）。该多重检测方案为多种食物风险因素提供了一种潜在的分析策略。