Department of Occupational Health and Safety Engineering, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Sci Rep. 2024 Oct 15;14(1):24161. doi: 10.1038/s41598-024-73010-w.
The need to identify ammonia is necessary because of its harmful effects on the environment and humans. In this study, a colorimetric method was also developed for the detection of ammonia using silver nanoparticles (AgNPs) synthesized with the green approach. Biosynthesis of AgNPs was performed by silver nitrate as a silver precursor and Smyrnium cordifolium extract as a reducing and stabilizing agent. Plant extract was studied by FTIR and LC/Mass techniques. The optimization of the effective parameters was carried out with central composite design according to silver nitrate concentration, plant extract volume, pH, and temperature. Biosynthetic nano-silver was characterized with XRD, EDS/EDX, FE-SEM, FTIR, TGA, and DLS methods. The AgNPs was validated for ammonia colorimetric detection. Biosynthesis of AgNPs were increased in 20 mM AgNO, 5 ml Smyrnium cordifolium extract, pH 10, and the temperature of 70 °C. Crystal form of AgNPs characterized with XRD at 2Ѳ value of 38.34°, 44.19°, 64.74°, and 77.59° and spherical shape highlighted in the range between 77.8 and 93 nm. Plant extract consisted of polyphenol (phenolic acid, flavonoid, and terpenoid), fatty acid, amino acid, sugar, purine, and organic acid. AgNPs were used for colorimetric detection of ammonia by shifting the λmax from 580 to 490 nm. A method for ammonia detection was set up, with linear range of 0.5-200 ppm, detection limit of 0.028 ppm and recovery level of 96.3 ± 6.5%. In conclusion, a new biosynthetic method by specified local plant was developed to propose a simple and sensitive colorimetric method for soluble ammonia detection.
由于氨对环境和人类的有害影响，因此需要对其进行鉴定。在这项研究中，还开发了一种使用通过绿色方法合成的银纳米粒子（AgNPs）检测氨的比色法。AgNPs 的生物合成是通过硝酸银作为银前体和 Smyrnium cordifolium 提取物作为还原和稳定剂来进行的。通过 FTIR 和 LC/MS 技术研究了植物提取物。根据硝酸银浓度、植物提取物体积、pH 值和温度，通过中心复合设计对有效参数进行了优化。通过 XRD、EDS/EDX、FE-SEM、FTIR、TGA 和 DLS 方法对生物合成纳米银进行了表征。AgNPs 被验证可用于氨的比色检测。在 20 mM AgNO、5 ml Smyrnium cordifolium 提取物、pH 10 和 70°C 的条件下，AgNPs 的生物合成增加。AgNPs 的晶体形态通过 XRD 在 2Ѳ 值为 38.34°、44.19°、64.74°和 77.59°处进行了表征，并在 77.8 至 93nm 的范围内呈现出球形。植物提取物由多酚（酚酸、类黄酮和萜烯）、脂肪酸、氨基酸、糖、嘌呤和有机酸组成。AgNPs 用于通过将 λmax 从 580nm 转移到 490nm 来进行氨的比色检测。建立了一种用于氨检测的方法，线性范围为 0.5-200ppm，检测限为 0.028ppm，回收率为 96.3±6.5%。总之，通过指定的当地植物开发了一种新的生物合成方法，提出了一种简单灵敏的比色法用于检测可溶性氨。
