Rogalsky V Y, Koven I H, Miller D R, Pollard A
Clin Chem. 1986 Jan;32(1 Pt 1):13-5.
We have studied the electrophoretic characteristics of a creatine kinase (CK, EC 2.7.3.2) isoenzyme, macro creatine kinase type 2 (MCK-2), in the serum of 42 patients with carcinoma of the colon. The patients could be divided into three subgroups by the intensity of the MCK-2 band (nine high, nine medium, and 24 low intensity), but we detected no correlation between the isoenzyme activity and the stage of the cancer. Inhibition of the activity of the CK-M subunit by antibody to CK-M allowed more sensitive detection of MCK-2 in serum and revealed that MCK-2 may be obscured by CK-MM. Moreover, there may be major discrepancies between the amount of MCK-2 seen on electrophoresis and the apparent total CK.
我们研究了42例结肠癌患者血清中一种肌酸激酶（CK，EC 2.7.3.2）同工酶——巨肌酸激酶2型（MCK-2）的电泳特征。根据MCK-2条带的强度，患者可分为三个亚组（九条高强度带、九条中等强度带和24条低强度带），但我们未检测到同工酶活性与癌症分期之间存在相关性。用抗CK-M抗体抑制CK-M亚基的活性，可更灵敏地检测血清中的MCK-2，并显示MCK-2可能被CK-MM掩盖。此外，电泳显示的MCK-2量与表观总CK之间可能存在重大差异。
