Caldini A L, Orlando C, Barni T, Messeri G, Pazzagli M, Baldi E, Serio M
Clin Chem. 1986 Jan;32(1 Pt 1):153-6.
We describe a luminescence immunoassay for measuring transferrin in human seminal plasma. Human transferrin conjugated with 7-[(N-4-aminobutyl)-N-ethylamino]naphthalene-1,2-dicarboxylic acid hydrazide was used to monitor the immunological reaction. The conjugate was stable for at least one year. The sensitivity (2 ng per tube) of the assay allows measurement of this protein in diluted seminal plasma. Results by this method (y) correlated well (r = .9681) with those by a conventional RIA method (x): y = 1.000x + 1.646 micrograms per ejaculate. Seminal transferrin concentrations are reported for normal control subjects, vasectomized subjects, and infertile patients. The method described appears suitable for measurement of seminal transferrin as an index of Sertoli cell function in male infertility.
我们描述了一种用于测量人类精浆中转铁蛋白的发光免疫测定法。与7-[(N-4-氨基丁基)-N-乙基氨基]萘-1,2-二羧酸酰肼偶联的人转铁蛋白用于监测免疫反应。该偶联物至少稳定一年。该测定法的灵敏度（每管2纳克）允许在稀释的精浆中测量这种蛋白质。该方法的结果（y）与传统放射免疫测定法（x）的结果相关性良好（r = 0.9681）：y = 1.000x + 1.646微克/射精量。报告了正常对照受试者、输精管结扎受试者和不育患者的精浆转铁蛋白浓度。所描述的方法似乎适用于测量精浆转铁蛋白，作为男性不育症中支持细胞功能的指标。
