Orlando C, Casano R, Forti G, Barni T, Vannelli G B, Balboni G C, Serio M
Department of Clinical Physiopathology, University of Florence, Italy.
J Reprod Fertil. 1988 Jul;83(2):687-92. doi: 10.1530/jrf.0.0830687.
An immunologically reactive albumin-like protein (albumin) was localized, by an immunostaining technique, in the testis of infertile men (normal spermatogenesis, obstructive azoospermia) at the level of the Sertoli cells and in some cells of the germinal epithelium (secondary spermatocytes and early spermatids). No positive reaction was detectable in prepubertal testis. In vasectomized men, mean seminal albumin values were drastically reduced (by about 80%) in comparison to fertile controls, indicating a probable testicular origin. Mean seminal albumin values were also decreased in patients affected by azoospermia due to a seminiferous tubular lesion (about 40%) and in oligozoospermic patients (about 30%). In the same seminal samples transferrin, an index of Sertoli cell function, was also measured. Albumin and transferrin results were well correlated in the seminal plasma of each group (with the exception of vasectomized subjects), including a group of men with abnormally high concentrations of seminal transferrin. A weak correlation was found between seminal albumin and sperm count. We suggest that the presence of albumin in the human adult testis and in seminal plasma could be related to its ability to transport androgens.
采用免疫染色技术，在不育男性（精子发生正常、梗阻性无精子症）睾丸的支持细胞水平以及生精上皮的一些细胞（次级精母细胞和早期精子细胞）中定位了一种具有免疫反应性的白蛋白样蛋白（白蛋白）。在青春期前睾丸中未检测到阳性反应。与生育对照组相比，输精管结扎男性的精液白蛋白平均水平大幅降低（约80%），表明其可能起源于睾丸。由于生精小管病变导致无精子症的患者以及少精子症患者的精液白蛋白平均水平也有所降低（约40%和约30%）。在相同的精液样本中，还检测了作为支持细胞功能指标的转铁蛋白。除输精管结扎受试者外，每组精液中的白蛋白和转铁蛋白结果相关性良好，其中包括一组精液转铁蛋白浓度异常高的男性。精液白蛋白与精子计数之间存在弱相关性。我们认为，成人睾丸和精液中白蛋白的存在可能与其运输雄激素的能力有关。
