Pilo A, Zucchelli G C, Chiesa M R, Bolelli G F, Albertini A
Clin Chem. 1986 Jan;32(1 Pt 1):171-4.
Data collected in a collaborative survey for radioimmunoassays have been studied by using analysis of variance to estimate the within-kit (CVw.kit) and the between-kit (CVb.kit) components of the total variability (CVT). This analysis has been applied to the results for triiodothyronine, thyroxin, thyrotropin, prolactin, and progesterone produced by 80-150 laboratories that assayed blind, replicate samples. Total variability was lowest in the thyroxin assay (CVT = 10.9%), associated with a very close between-kit agreement (CVb.kit = 4.0%); in the triiodothyronine assay, on the other hand, the large between-kit component (CVb.kit = 10.1%) increased the total variability to 16.1%. In the prolactin assay the CVT of 19.3% included 17.5% CVw.kit and 8.1% CVb.kit. Assays for thyrotropin and progesterone involve analyses of two pools at different hormone concentrations. The CVb.kit component was very high in the low-concentration pool, both for thyrotropin (25.1%) and progesterone (45.2%); in the higher-concentration pool it decreased to 8.3% for thyrotropin but remained high (21.6%) for progesterone. Applying analysis of variance to the triiodothyronine and thyroxin data obtained by different laboratories using the same kit showed that most kits yielded significantly different measurements when used in different laboratories.
在一项放射免疫分析协作调查中收集的数据，已通过方差分析进行研究，以估计总变异性（CVT）的试剂盒内（CVw.kit）和试剂盒间（CVb.kit）成分。该分析已应用于80 - 150个实验室对三碘甲状腺原氨酸、甲状腺素、促甲状腺激素、催乳素和孕酮进行盲法检测重复样本的结果。甲状腺素检测的总变异性最低（CVT = 10.9%），试剂盒间一致性非常高（CVb.kit = 4.0%）；另一方面，在三碘甲状腺原氨酸检测中，较大的试剂盒间成分（CVb.kit = 10.1%）使总变异性增加到16.1%。在催乳素检测中，19.3%的CVT包括17.5%的CVw.kit和8.1%的CVb.kit。促甲状腺激素和孕酮检测涉及对不同激素浓度的两个样本池进行分析。在低浓度样本池中，促甲状腺激素（25.1%）和孕酮（45.2%）的CVb.kit成分都非常高；在高浓度样本池中，促甲状腺激素的该成分降至8.3%，但孕酮仍很高（21.6%）。对不同实验室使用同一试剂盒获得的三碘甲状腺原氨酸和甲状腺素数据进行方差分析表明，大多数试剂盒在不同实验室使用时产生的测量结果有显著差异。
