Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40-032 Katowice, Poland.
Polish Academy of Sciences, Institute of Ichthyobiology and Aquaculture in Gołysz, 43-520 Zaborze, Poland.
Molecules. 2024 Sep 27;29(19):4594. doi: 10.3390/molecules29194594.
The use of nanoparticles in the industry carries the risk of their release into the environment. Based on the presumption that the primary graphene oxide (GO) toxicity mechanism is reactive oxygen species production in the cell, the question arises as to whether well-known antioxidants can protect the cell or significantly reduce the effects of GO. This study focused on the possible remedial effect of vitamin C in intoxicated with GO for whole lives. The reproduction potential was measured at the level of Vitellogenin () gene expression, Vg protein expression, hatching success, and share of nutrition in the developing egg. There was no simple relationship between the gene's expression and the Vg protein content. Despite fewer eggs laid in the vitamin C groups, hatching success was high, and egg composition did not differ significantly. The exceptions were GO20 and GO20 + Vit. C groups, with a shift in the lipid content in the egg. Most likely, ascorbic acid impacts the level of gene expression but does not affect the production of Vg protein or the quality of eggs laid. Low GO concentration in food did not cause adverse effects, but the relationship between GO toxicity and its concentration should be investigated more thoroughly.
纳米粒子在工业中的应用存在着释放到环境中的风险。基于这样一种假设，即主要的氧化石墨烯 (GO) 毒性机制是细胞内活性氧物质的产生，那么问题就出现了，即是否已知的抗氧化剂可以保护细胞或显著降低 GO 的影响。本研究关注的是维生素 C 在一生中持续摄入 GO 时的可能补救效果。繁殖潜力是通过 Vitellogenin () 基因表达、Vg 蛋白表达、孵化成功率和发育卵中营养物质的比例来衡量的。基因表达和 Vg 蛋白含量之间没有简单的关系。尽管维生素 C 组产卵较少，但孵化成功率很高，而且卵的组成没有显著差异。GO20 和 GO20+Vit.C 组是例外，其卵中的脂质含量发生了转移。很可能，抗坏血酸会影响基因表达的水平，但不会影响 Vg 蛋白的产生或产卵的质量。食物中低浓度的 GO 没有引起不良反应，但 GO 毒性与其浓度之间的关系应该更深入地研究。
