Dziewięcka Marta, Pawlyta Mirosława, Majchrzycki Łukasz, Balin Katarzyna, Barteczko Sylwia, Czerkawska Martyna, Augustyniak Maria
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland.
Department of Engineering Materials and Biomaterials, Faculty of Mechanical Engineering, Silesian University of Technology, Konarskiego 18A, 44-100 Gliwice, Poland.
Int J Mol Sci. 2021 May 20;22(10):5401. doi: 10.3390/ijms22105401.
Interest in graphene oxide nature and potential applications (especially nanocarriers) has resulted in numerous studies, but the results do not lead to clear conclusions. In this paper, graphene oxide is obtained by multiple synthesis methods and generally characterized. The mechanism of GO interaction with the organism is hard to summarize due to its high chemical activity and variability during the synthesis process and in biological buffers' environments. When assessing the biocompatibility of GO, it is necessary to take into account many factors derived from nanoparticles (structure, morphology, chemical composition) and the organism (species, defense mechanisms, adaptation). This research aims to determine and compare the in vivo toxicity potential of GO samples from various manufacturers. Each GO sample is analyzed in two concentrations and applied with food. The physiological reactions of an easy model (cell viability, apoptosis, oxidative defense, DNA damage) during ten-day lasting exposure were observed. This study emphasizes the variability of the GO nature and complements the biocompatibility aspect, especially in the context of various GO-based experimental models. Changes in the cell biomarkers are discussed in light of detailed physicochemical analysis.
对氧化石墨烯的性质及其潜在应用（尤其是纳米载体）的关注引发了大量研究，但结果并未得出明确结论。在本文中，通过多种合成方法制备了氧化石墨烯并对其进行了一般表征。由于氧化石墨烯在合成过程中以及在生物缓冲液环境中的高化学活性和变异性，其与生物体相互作用的机制难以总结。在评估氧化石墨烯的生物相容性时，有必要考虑许多源自纳米颗粒（结构、形态、化学成分）和生物体（物种、防御机制、适应性）的因素。本研究旨在确定并比较不同制造商生产的氧化石墨烯样品的体内潜在毒性。每种氧化石墨烯样品均以两种浓度进行分析，并与食物一起施用。观察了在为期十天的暴露期间简单模型（细胞活力、凋亡、氧化防御、DNA损伤）的生理反应。本研究强调了氧化石墨烯性质的变异性，并补充了生物相容性方面的内容，特别是在各种基于氧化石墨烯的实验模型的背景下。根据详细的物理化学分析讨论了细胞生物标志物的变化。
