College of Agronomy, Anhui Science and Technology University, Chuzhou 233100, China.
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
Int J Mol Sci. 2024 Sep 24;25(19):10264. doi: 10.3390/ijms251910264.
Carotenoid cleavage oxygenases (CCOs) cleave carotenoid molecules to produce bioactive products that influence the synthesis of hormones such as abscisic acid (ABA) and strigolactones (SL), which regulate plant growth, development, and stress adaptation. Here, to explore the molecular characteristics of all members of the OsCCO family in rice, fourteen family genes were identified in the genome-wide study. The results revealed that the OsCCO family included one OsNCED and four OsCCD subfamilies. The OsCCO family was phylogenetically close to members of the maize ZmCCO family and the SbCCO family. A collinearity relationship was observed between and in rice, as well as and between rice and , and maize. OsCCD4a and OsCCD7 were the key members in the protein interaction network of the OsCCO family, which was involved in the catabolic processes of carotenoids and terpenoid compounds. miRNAs targeting family members were mostly involved in the abiotic stress response, and RNA-seq data further confirmed the molecular properties of family genes in response to abiotic stress and hormone induction. qRT-PCR analysis showed the differential expression patterns of members across various rice organs. Notably, showed relatively high expression levels in all organs except for ripening seeds and endosperm. , , , , , , and were potentially involved in plant growth and differentiation. Meanwhile, , , , , and were associated with reproductive organ development, flowering, and seed formation. , , , , and were related to assimilate transport and seed maturation. These findings provide a theoretical basis for further functional analysis of the OsCCO family.
类胡萝卜素裂解加氧酶（CCOs）裂解类胡萝卜素分子，产生影响激素如脱落酸（ABA）和独脚金内酯（SL）合成的生物活性产物，这些激素调节植物的生长、发育和应激适应。在这里，为了探索水稻中 OsCCO 家族所有成员的分子特征，在全基因组研究中鉴定了 14 个家族基因。结果表明，OsCCO 家族包括一个 OsNCED 和四个 OsCCD 亚家族。OsCCO 家族在系统发育上与玉米 ZmCCO 家族和 SbCCO 家族的成员关系密切。在水稻中观察到 和 之间存在共线性关系，以及水稻和 之间、 和 之间存在共线性关系。OsCCD4a 和 OsCCD7 是 OsCCO 家族蛋白互作网络中的关键成员，该网络参与了类胡萝卜素和萜烯化合物的分解代谢过程。靶向 家族成员的 miRNAs 主要参与非生物胁迫响应，RNA-seq 数据进一步证实了 家族基因在应对非生物胁迫和激素诱导方面的分子特性。qRT-PCR 分析显示， 家族成员在各种水稻器官中的表达模式存在差异。值得注意的是， 除了成熟种子和胚乳外，在所有器官中均表现出相对较高的表达水平。,,,,,, 和 可能参与植物生长和分化。同时，,,,, 和 与生殖器官发育、开花和种子形成有关。,,,, 和 与同化产物运输和种子成熟有关。这些发现为进一步研究 OsCCO 家族的功能提供了理论依据。
