Li Huan, Yao Xin, He Ailing, Xue Guoxing, Yang Haizhu, Fan Yu, Yang Sanwei, Ruan Jingjun
College of Agriculture, Guizhou University, Guiyang, Guiyang, Guizhou, 550025, China.
College of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, 610106, China.
BMC Plant Biol. 2025 Apr 12;25(1):466. doi: 10.1186/s12870-025-06503-z.
Carotenoid cleavage oxygenases (CCOs) convert carotenoids into volatile aromatic compounds implicated in plant growth and development. They affect the synthesis of hormones, including abscisic acid (ABA) and strigolactone (SL). However, the CCO family in Tartary buckwheat remains unelucidated.
We identified the FtCCO gene family based on Tartary buckwheat genomic data and analyzed the biological function of the FtCCO genes using bioinformatics methods and the expression pattern of the gene using fluorescence quantitative PCR. Three pairs of fragment duplication genes were found in FtCCOs, and the motifs were highly conserved within the same subfamily. FtCCO genes are closely related to the dicotyledonous Arabidopsis thaliana, which has the highest number of co-linear genes. The qRT-PCR showed that among the tissue-specific expression patterns of Tartary buckwheat CCO genes, the expression of the FtCCOs was higher in the leaves. In Tartary buckwheat grain development, the relative expression of most FtCCOs was higher at the later stage. The relative expression of many genes was higher in the stems under cold, dark, NaCl, and abiotic stress conditions. However, under the hormone and plant growth regulator treatments, the expression of the nine FtCCOs was relatively low in the stems. Notably, the relative expression of FtNCED4 was extremely high under abiotic stress and hormone induction, indicating that FtNCED4 may be involved in the growth and development of Tartary buckwheat. In this study, the FtCCO family genes of Tartary buckwheat were identified at the genome-wide level, and the gene expression pattern of the FtCCO gene family in different tissues or treatments was determined. This study provides a theoretical basis for further analysis of the functions of theFtCCO family, which is of great significance for the mining of resistance genes and trait improvement.
类胡萝卜素裂解双加氧酶（CCOs）将类胡萝卜素转化为与植物生长发育相关的挥发性芳香化合物。它们影响包括脱落酸（ABA）和独脚金内酯（SL）在内的激素合成。然而，苦荞中的CCO家族仍未阐明。
我们基于苦荞基因组数据鉴定了FtCCO基因家族，并使用生物信息学方法分析了FtCCO基因的生物学功能，同时通过荧光定量PCR分析了该基因的表达模式。在FtCCOs中发现了三对片段重复基因，且同一亚家族内的基序高度保守。FtCCO基因与双子叶植物拟南芥密切相关，拟南芥具有最多的共线基因。qRT-PCR结果显示，在苦荞CCO基因的组织特异性表达模式中，FtCCOs在叶片中的表达较高。在苦荞籽粒发育过程中，大多数FtCCOs的相对表达在后期较高。在寒冷、黑暗、NaCl和非生物胁迫条件下，许多基因在茎中的相对表达较高。然而，在激素和植物生长调节剂处理下，九个FtCCOs在茎中的表达相对较低。值得注意的是，FtNCED4在非生物胁迫和激素诱导下的相对表达极高，表明FtNCED4可能参与苦荞的生长发育。在本研究中，在全基因组水平上鉴定了苦荞的FtCCO家族基因，并确定了FtCCO基因家族在不同组织或处理中的基因表达模式。本研究为进一步分析FtCCO家族的功能提供了理论依据，对抗性基因挖掘和性状改良具有重要意义。
