ICAR-Indian Institute of Maize Research, Ludhiana 141004, India.
ICAR-Indian Agricultural Research Institute Regional Station, Karnal 132001, India.
Int J Mol Sci. 2024 Sep 25;25(19):10300. doi: 10.3390/ijms251910300.
Maize is a highly versatile crop holding significant importance in global food, feed and nutritional security. Grain yield is a complex trait and difficult to improve without targeting the improvement of grain yield attributing traits, which are relatively less complex in nature. Hence, considering the erosion in genetic diversity, there is an urgent need to use wild relatives for genetic diversification and unravel the genomic regions for grain yield attributing traits in maize. Thus, the current study aimed to identify quantitative trait loci (QTLs) linked with grain yield and yield attributing traits. Two BCF populations developed from the cross of LM13 with (population 1) and LM14 with (population 2) were genotyped and phenotyped in field conditions in the kharif season. BCF lines in both populations were phenotyped again for grain yield and attributing traits in the spring season. In total, three QTLs each for ear height (EH), two QTLs for flag leaf length (FLL) and one QTL each for ear diameter (ED), plant height, flag leaf length (FLL), flag leaf width and 100 kernel-weight were identified in population 1. In population 2, two QTLs for kernel row per ear (KRPE) and one QTL for FLL were detected in. QTLs for EH, FLL and KPRE showed consistency across seasons. Among the identified QTLs, six QTLs were found to be co-localized near identified genomic regions in previous studies, validating their potential in contributing to trait expression. The identified QTLs can be utilized for marker assisted selection, transferring favorable alleles from wild relatives in modern maize.
玉米是一种用途广泛的作物，在全球粮食、饲料和营养安全方面具有重要意义。籽粒产量是一个复杂的性状，如果不针对提高与产量相关的性状进行改良，就很难提高产量，而这些性状在本质上相对不太复杂。因此，考虑到遗传多样性的侵蚀，迫切需要利用野生近缘种进行遗传多样化，并阐明玉米产量相关性状的基因组区域。因此，本研究旨在鉴定与籽粒产量和产量相关性状相关的数量性状位点（QTL）。从 LM13 与 （群体 1）和 LM14 与 （群体 2）杂交产生的两个 BCF 群体在雨季的田间条件下进行了基因型和表型分析。在春季，两个群体中的 BCF 系再次对籽粒产量和相关性状进行了表型分析。总共在群体 1 中鉴定到了 3 个与穗高（EH）相关的 QTL、2 个与旗叶长度（FLL）相关的 QTL 和 1 个与穗径（ED）、株高、旗叶长度（FLL）、旗叶宽度和 100 粒重相关的 QTL。在群体 2 中，鉴定到了 2 个与每穗粒行数（KRPE）相关的 QTL 和 1 个与 FLL 相关的 QTL。EH、FLL 和 KPRE 的 QTL 在不同季节具有一致性。在所鉴定的 QTL 中，有 6 个 QTL 被发现在先前研究中鉴定到的基因组区域附近存在共定位，这验证了它们在促进性状表达方面的潜力。所鉴定的 QTL 可用于标记辅助选择，将有利的等位基因从野生近缘种转移到现代玉米中。
