Department of Electroradiology, Poznan University of Medical Sciences, 61-701 Poznan, Poland.
Doctoral School, Poznan University of Medical Sciences, 61-701 Poznan, Poland.
Int J Mol Sci. 2024 Sep 29;25(19):10494. doi: 10.3390/ijms251910494.
Research on different types of ionizing radiation's effects has been ongoing for years, revealing its efficacy in damaging cancer cells. Solid tumors comprise diverse cell types, each being able to respond differently to radiation. This study evaluated the radiobiological response of established (MDA-MB-231 (Triple negative breast cancer, TNBC), MCF-7 (Luminal A)) and patient-derived malignant cell lines, cancer-associated fibroblasts, and skin fibroblasts following proton IRR. All cell line types were irradiated with the proton dose of 2, 4, and 6 Gy. The radiobiological response was assessed using clonogenic assay, γH2AX, and p53 staining. It was noticeable that breast cancer lines of different molecular subtypes displayed no significant variations in their response to proton IRR. In terms of cancer-associated fibroblasts extracted from the tumor tissue, the line derived from a TNBC subtype tumor demonstrated higher resistance to ionizing radiation compared to lines isolated from luminal A tumors. Fibroblasts extracted from patients' skin responded identically to all doses of proton radiation. This study emphasizes that tumor response is not exclusively determined by the elimination of breast cancer cells, but also takes into account tumor microenvironmental variables and skin reactions.
多年来，对不同类型电离辐射效应的研究一直在进行，揭示了其破坏癌细胞的功效。实体瘤由多种细胞类型组成，每种细胞对辐射的反应都不同。本研究评估了在质子辐照后，已建立的（MDA-MB-231（三阴性乳腺癌，TNBC），MCF-7（Luminal A））和患者来源的恶性细胞系、癌相关成纤维细胞和皮肤成纤维细胞的放射生物学反应。所有细胞系类型均用 2、4 和 6 Gy 的质子剂量照射。使用集落形成测定、γH2AX 和 p53 染色评估放射生物学反应。值得注意的是，不同分子亚型的乳腺癌细胞系对质子辐照的反应没有明显差异。就从肿瘤组织中提取的癌相关成纤维细胞而言，源自 TNBC 亚型肿瘤的细胞系对电离辐射的抵抗力高于从 Luminal A 肿瘤中分离的细胞系。从患者皮肤中提取的成纤维细胞对所有质子辐射剂量的反应相同。这项研究强调，肿瘤反应不仅取决于乳腺癌细胞的消除，还考虑到肿瘤微环境变量和皮肤反应。
