Lach Michał Stefan, Wróblewska Joanna Patrycja, Michalak Marcin, Budny Bartłomiej, Wrotkowska Elżbieta, Suchorska Wiktoria Maria
Department of Electroradiology, Poznan University of Medical Sciences, Garbary 15, 61-866 Poznan, Poland.
Radiobiology Lab, The Greater Poland Cancer Centre, Garbary 15 Street, 61-866 Poznan, Poland.
Int J Mol Sci. 2025 Jan 4;26(1):376. doi: 10.3390/ijms26010376.
The vast majority of breast cancer patients require radiotherapy but some of them will develop local recurrences and potentially metastases in the future. Recent data show that exosomal cargo is essential in these processes. Thus, we investigated the influence of ionising radiation on exosome properties and their ability to modify the sensitivity and biology of non-irradiated cells. Exosomes were isolated from breast cancer cell lines (MDA-MB-231, MCF7, and SKBR3) irradiated with 2 Gy (Exo 2 Gy) or no irradiation (Exo 0 Gy). Despite some differences in their molecular profiles, they did not affect cell viability, proliferation, cell cycle phase distribution, and radioresistance; however, both populations showed the ability to modify cell migration and invasion potential, as confirmed by the downregulation of β-catenin, which is responsible for maintaining the epithelial phenotype. Interestingly, exosomes from irradiated BCa cells were more actively deposited in the endothelial cells (EA.hy926). Furthermore, exosomes tend to lower the expression of CD31, which is responsible for maintaining intact vascularity. This preliminary study demonstrates the vital role of exosomes and their altered profile due to irradiation in the pathobiology of breast cancer.
绝大多数乳腺癌患者需要放疗，但其中一些患者将来会出现局部复发并可能发生转移。最近的数据表明，外泌体成分在这些过程中至关重要。因此，我们研究了电离辐射对外泌体特性及其改变未受辐射细胞敏感性和生物学特性能力的影响。从接受2 Gy照射（Exo 2 Gy）或未照射（Exo 0 Gy）的乳腺癌细胞系（MDA-MB-231、MCF7和SKBR3）中分离外泌体。尽管它们的分子谱存在一些差异，但它们并未影响细胞活力、增殖、细胞周期阶段分布和放射抗性；然而，这两种外泌体群体均显示出改变细胞迁移和侵袭潜能的能力，这一点通过下调负责维持上皮表型的β-连环蛋白得到证实。有趣的是，来自受照射乳腺癌细胞的外泌体更活跃地沉积在内皮细胞（EA.hy926）中。此外，外泌体倾向于降低负责维持完整血管的CD31的表达。这项初步研究证明了外泌体及其因辐射而改变的特征在乳腺癌病理生物学中的重要作用。
