Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46# East of Construction Road, Xinxiang 453007, China.
Int J Mol Sci. 2024 Sep 30;25(19):10542. doi: 10.3390/ijms251910542.
Since Teleostei fins have a strong regenerative capacity, further research was conducted on the regulation of gene expression during fin regeneration. This research focuses on miRNA, which is a key post-transcriptional regulatory molecule. In this study, a miRNA library for the fin regeneration of zebrafish was constructed to reveal the differential expression of miRNA during fin regeneration and to explore the regulatory pathway for fin regeneration. Following the injection of miRNA agomir into zebrafish, the proliferation of blastema cells and the overall fin regeneration area were significantly reduced. It was observed that the miRNAs impaired blastocyte formation by affecting fin regeneration through the inhibition of the expressions of genes and proteins associated with blastocyte formation (including and Smad1/5/9), which is an effect associated with the Hippo pathway. Furthermore, it has been demonstrated that miRNAs can impair the patterns and mineralization of newly formed fin rays. The miRNAs influenced fin regeneration by inhibiting the expression of a range of bone-related genes and proteins in osteoblast lineages, including , , and This study provides a valuable reference for the further exploration of morphological bone reconstruction in aquatic vertebrates.
由于硬骨鱼类的鳍具有很强的再生能力，因此进一步研究了基因表达在鳍再生过程中的调控。这项研究的重点是 miRNA，它是一种关键的转录后调控分子。在这项研究中，构建了一个用于斑马鱼鳍再生的 miRNA 文库，以揭示 miRNA 在鳍再生过程中的差异表达，并探讨鳍再生的调控途径。向斑马鱼注射 miRNA agomir 后，胚细胞的增殖和整个鳍再生区域显著减少。观察到 miRNA 通过抑制与胚细胞形成相关的基因和蛋白质的表达（包括 和 Smad1/5/9）来影响胚细胞的形成，从而损害胚细胞的形成，这是与 Hippo 途径相关的作用。此外，已经证明 miRNA 可以损害新形成的鳍射线的模式和矿化。miRNA 通过抑制成骨细胞谱系中一系列与骨相关的基因和蛋白质的表达来影响鳍的再生，包括 、 、 和 。本研究为进一步探索水生脊椎动物形态骨重建提供了有价值的参考。
