Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA.
Int J Mol Sci. 2024 Oct 2;25(19):10618. doi: 10.3390/ijms251910618.
Insecticide resistance in insects, driven by the overexpression of P450 enzymes, presents a significant challenge due to the enhanced metabolic detoxification of insecticides. Although the transcriptional regulation of P450 genes is not yet fully understood, G-protein-coupled receptor (GPCR) genes have emerged as key regulators in this process. This study is the first to associate GPCR genes with insecticide resistance in . We identified two key rhodopsin-like GPCR genes, and , which were significantly overexpressed in the resistant ALHF strain compared to sensitive strains. Notably, both and were mapped to autosome 2, where critical but unidentified regulatory factors controlling resistance and P450 gene regulation are located. This supports our hypothesis that GPCRs function as trans-regulatory factors for P450-mediated resistance. Functional analysis using transgenic demonstrated that overexpression of these rhodopsin-like GPCR genes increased permethrin resistance by approximately two-fold. Specifically, overexpression significantly upregulated the resistance-related P450 genes , , and , while increased and expression, thereby enhancing insecticide detoxification in rhodopsin-like GPCR transgenic lines. These findings suggest that these rhodopsin-like GPCR genes on autosome 2 may act as trans-regulatory factors for P450-mediated resistance, underscoring their critical role in insecticide detoxification and resistance development in .
昆虫体内的杀虫剂抗性，由于 P450 酶的过度表达，导致杀虫剂的代谢解毒增强，这是一个重大挑战。尽管 P450 基因的转录调控尚未完全了解，但 G 蛋白偶联受体 (GPCR) 基因已成为该过程的关键调节因子。本研究首次将 GPCR 基因与 中的杀虫剂抗性联系起来。我们鉴定了两个关键的视蛋白样 GPCR 基因 和 ，它们在抗性 ALHF 株中相对于敏感株显著过表达。值得注意的是， 和 都被映射到 2 号染色体上，该染色体上存在控制抗性和 P450 基因调控的关键但尚未确定的调节因子。这支持了我们的假设，即 GPCR 作为 P450 介导的抗性的转录调节因子发挥作用。使用转基因 的功能分析表明，这些视蛋白样 GPCR 基因的过表达使拟除虫菊酯抗性增加了约两倍。具体来说， 过表达显著上调了与 抗性相关的 P450 基因 、 和 ，而 增加了 和 的表达，从而增强了视蛋白样 GPCR 转基因 系中的杀虫剂解毒作用。这些发现表明，2 号染色体上的这些视蛋白样 GPCR 基因可能作为 P450 介导的抗性的转录调节因子，突出了它们在 中杀虫剂解毒和抗性发展中的关键作用。
