Zhu F, Feng J-N, Zhang L, Liu N
Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, USA.
Insect Mol Biol. 2008 Feb;17(1):27-37. doi: 10.1111/j.1365-2583.2008.00777.x.
Two novel P450 cDNAs, CYP6A36 and CYP6A37, were isolated from house-flies. Putative protein sequences deduced from the cDNA sequences shared 58% identity. Predicted protein sequences of CYP6A36 and CYP6A37 from pyrethroid-resistant ALHF house-flies were identical to their corresponding orthologues in susceptible aabys flies. Expression of CYP6A36 was developmentally regulated with significant overexpression in ALHF compared with susceptible CS flies. Overexpression of CYP6A36 was detected in ALHF abdomen, where the primary detoxification organs of the midgut and fat body are located. CYP6A37, however, was expressed in all tested life stages, with no significant difference in expression between ALHF and CS. Genetic linkage analysis located CYP6A36 on autosome 5; overexpression of CYP6A36 was linked to the factors on autosomes 1 and 2, corresponding to the linkage of P450-mediated resistance in ALHF. This evidence suggests the importance of CYP6A36 in detoxification of insecticides and evolution of insecticide resistance in ALHF.
从家蝇中分离出两个新的细胞色素P450 cDNA，即CYP6A36和CYP6A37。从cDNA序列推导的假定蛋白质序列具有58%的同一性。来自抗拟除虫菊酯的ALHF家蝇的CYP6A36和CYP6A37的预测蛋白质序列与其在敏感的aabys家蝇中的相应直系同源物相同。CYP6A36的表达受发育调控，与敏感的CS家蝇相比，在ALHF中显著过表达。在ALHF腹部检测到CYP6A36过表达，中肠和脂肪体的主要解毒器官位于该部位。然而，CYP6A37在所有测试的生命阶段均有表达，ALHF和CS之间的表达无显著差异。遗传连锁分析将CYP6A36定位在5号常染色体上；CYP6A36的过表达与1号和2号常染色体上的因子相关，这与ALHF中P450介导的抗性连锁相对应。这一证据表明CYP6A36在ALHF中杀虫剂解毒和抗药性进化中的重要性。
