College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.
Int J Mol Sci. 2024 Oct 5;25(19):10724. doi: 10.3390/ijms251910724.
The ATP-binding cassette (ABC) proteins are a diverse family of transmembrane transporter proteins widely identified in various organisms. The ABCG transporters belong to the G subfamily of the ABC transporter family. Rarely research on ABCG transporters involved in salt tolerance of rice was found. In this study, the evolutionary relationships, conserved motifs, intra- and inter-species homologous genes, and cis-acting elements of ABCG subfamily members were analyzed, and the expression changes of these genes under salt stress at 0 h, 3 h, and 24 h were detected. Based on these results, the candidate gene , which is induced by salt stress, was selected for further studies. Yeast experiments confirmed that the gene might be involved in the regulation of salt tolerance. The abcg7 mutant showed a higher degree of leaf wilting and a lower survival rate, exhibiting a salt-sensitive phenotype. Systematic analysis of this family in rice helps design effective functional analysis strategies and provides data support for understanding the role of ABCG transporters under salt stress.
三磷酸腺苷结合盒（ABC）蛋白是一类广泛存在于各种生物中的跨膜转运蛋白超家族。ABCG 转运蛋白属于 ABC 转运蛋白家族的 G 亚家族。很少有研究涉及 ABCG 转运蛋白在水稻耐盐性中的作用。本研究分析了 ABCG 亚家族成员的进化关系、保守基序、种内和种间同源基因以及顺式作用元件，并检测了这些基因在 0 h、3 h 和 24 h 盐胁迫下的表达变化。基于这些结果，选择了候选基因 ，该基因受盐胁迫诱导，进一步研究。酵母实验证实该基因可能参与盐胁迫的调节。abcg7 突变体表现出较高的叶片萎蔫程度和较低的存活率，表现出盐敏感表型。对水稻中该家族的系统分析有助于设计有效的功能分析策略，并为理解 ABCG 转运蛋白在盐胁迫下的作用提供数据支持。
