Indian Institute of Technology Roorkee, India.
Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
Plant Physiol Biochem. 2019 Sep;142:429-439. doi: 10.1016/j.plaphy.2019.08.004. Epub 2019 Aug 8.
ABC transporters constitute the largest family of transporter proteins in living organisms and divided into eight subfamilies, from A-H. ABCG members, specific to plants and fungi, belong to subfamily G. In this study, we provide updated inventory, detailed account of phylogeny, gene structure characteristics, and expression profiling during reproductive development, abiotic and biotic stresses of members of ABCG gene family in rice along with reannotation and cloning of FL-cDNA of OsABCG50/PDR23. We observed that of the 22 ABCGs/PDRs, four genes evolved as a result of gene duplication events and their expression pattern changed after duplication. Analysis of expression revealed seed and developmental stage preferential expression of five ABCG/PDR members. Transcript levels of eight ABCGs/PDRs were affected by abiotic and biotic stresses. Expression of seven ABCG/PDR genes was also altered by hormonal elicitors. The modulated expression is nicely correlated with the presence of tissue/stress specific cis-acting elements present in putative promoter region.
ABC 转运蛋白构成了生物体中最大的转运蛋白家族，分为 A-H 八个亚家族。ABC 转运蛋白 G 成员是植物和真菌特有的，属于亚家族 G。在这项研究中，我们提供了水稻 ABCG 基因家族成员的最新清单、系统发育的详细说明、基因结构特征以及生殖发育、非生物和生物胁迫过程中的表达谱，同时还对 OsABCG50/PDR23 的 FL-cDNA 进行了重新注释和克隆。我们观察到，在 22 个 ABCG/PDR 中，有 4 个基因是由于基因复制事件而进化的，并且它们的表达模式在复制后发生了变化。表达分析表明，五个 ABCG/PDR 成员在种子和发育阶段具有偏好性表达。八种 ABCG/PDR 的转录水平受到非生物和生物胁迫的影响。七种 ABCG/PDR 基因的表达也受到激素诱导剂的改变。调节表达与假定启动子区域中存在的组织/胁迫特异性顺式作用元件很好地相关。
