Chen Zhilei, Cao Hao, Jin Jing, Li Zhong, Zhang Shouke, Chen Jie
Zhejiang Green Pesticide 2011 Collaborative Innovation Center, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Zhejiang Tonglu Huifeng Biosciences Co., Ltd., Hangzhou 311500, China.
Plants (Basel). 2024 Sep 30;13(19):2743. doi: 10.3390/plants13192743.
Anthracnose, a fungal disease harming fruit trees and crops, poses a threat to agriculture. Traditional chemical pesticides face issues like environmental pollution and resistance. A strategy combining low-toxicity chemicals with biopesticides is proposed to enhance disease control while reducing chemical use. Our study found that mixing validamycin A (VMA) and TCS001 effectively controlled anthracnose in . The combination increased antifungal efficacy by 65.62% over VMA alone and 18.83% over TCS001 alone. It caused pathogen deformities and loss of pathogenicity. Transcriptomic analysis revealed that the mix affected the pathogen's metabolism and redox processes, particularly impacting cellular membrane functions and inducing apoptosis via glycolysis/gluconeogenesis. In vivo tests showed the treatment activated 's disease resistance, with a 161.72% increase in polyphenol oxidase concentration in treated plants. This research offers insights into VMA and TCS001's mechanisms against anthracnose, supporting sustainable forestry and national edible oil security.
炭疽病是一种危害果树和农作物的真菌病害，对农业构成威胁。传统化学农药面临环境污染和抗性等问题。提出了一种将低毒化学药剂与生物农药相结合的策略，以在减少化学药剂使用的同时加强病害防治。我们的研究发现，井冈霉素A（VMA）和TCS001混合使用能有效防治[具体对象未提及]中的炭疽病。该组合比单独使用VMA的抗真菌效果提高了65.62%，比单独使用TCS001提高了18.83%。它导致病原体畸形并丧失致病性。转录组分析表明，该混合物影响了病原体的代谢和氧化还原过程，尤其影响细胞膜功能，并通过糖酵解/糖异生诱导细胞凋亡。体内试验表明，该处理激活了[具体对象未提及]的抗病性，处理后的植株中多酚氧化酶浓度提高了161.72%。本研究为VMA和TCS001防治炭疽病的作用机制提供了见解，支持了可持续林业和国家食用油安全。
