National Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Zhejiang Tongchuang Space Technology Co ltd., Hangzhou 311300, China.
J Agric Food Chem. 2024 Aug 7;72(31):17405-17416. doi: 10.1021/acs.jafc.4c03323. Epub 2024 Jul 23.
is an important fungal pathogen that causes gray mold disease in plants. Previously, TCS001 live culture presented broad-spectrum antifungal activity against various plant pathogenic fungi and oomycetes, particularly . Here, the bioactivity of lipopeptides produced by TCS001 against was investigated. The IC values of the crude lipopeptide extract (CLE) from TCS001 to suppress mycelial growth and conidial germination were 14.20 and 49.39 mg/L, respectively. SEM and TEM imaging revealed that CLE caused morphological deformities and ultrastructural changes in the mycelium. Transcriptomic analyses combined with Δ mutant construction demonstrated that the CLE could confer antifungal activity via suppressing expression in the pathogen. In addition, the CLE activated the plant immune system by increasing the content of defense-related enzymes and the expression of marker genes in immunity signaling pathways in cucumber plants. Therefore, TCS001 CLE could be potentially developed into biopesticides for the biocontrol of gray mold disease.
是一种重要的真菌病原体，可引起植物灰霉病。先前，TCS001 活菌培养物对各种植物病原真菌和卵菌表现出广谱的抗真菌活性，特别是 。在这里，研究了 TCS001 产生的脂肽对 的生物活性。TCS001 产生的粗脂肽提取物（CLE）抑制菌丝生长和分生孢子萌发的 IC 值分别为 14.20 和 49.39mg/L。SEM 和 TEM 成像显示，CLE 导致菌丝形态畸形和超微结构改变。转录组分析结合 Δ突变体构建表明，CLE 可以通过抑制病原菌中 的表达来赋予抗真菌活性。此外，CLE 通过增加黄瓜植物中防御相关酶的含量和免疫信号通路标记基因的表达来激活植物免疫系统。因此，TCS001 CLE 有可能被开发成防治灰霉病的生物农药。
