Korotaeva Natalia, Shmakov Vladimir, Bel'kov Vadim, Pyatrikas Daria, Moldavskaya Sofia, Gorbenko Igor
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, Irkutsk 664033, Russia.
Plants (Basel). 2024 Sep 30;13(19):2752. doi: 10.3390/plants13192752.
Under a water deficit, the protective proteins known as dehydrins (DHNs) prevent nonspecific interactions in protein and membrane structures and their damage, in addition to playing an antioxidant role. The DHNs of a widespread xerophytic species Scots pine ( L.) have been poorly studied, and their role in resistance to water deficits has not been revealed. In this paper, we have expanded the list of DHNs that accumulate in the cells of Scots pine under the conditions of water deficits and revealed their relationship with the effects of water deficits. In this investigation, callus cultures of branches and buds of Scots pine were used. A weak water deficit was created by adding polyethylene glycol to the culture medium. Under the conditions of a water deficit, the activity of catalase and peroxidase enzymes increased in the callus cultures. A moderate decrease in the total water content was correlated with a decrease in the growth rate of the callus cultures, as well as with an increase in the activity of lipid peroxidation. The accumulation of Mr 72, 38, and 27 kDa DHNs occurred in the callus cultures of buds, and the accumulation of Mr 72 and 27 kDa DHNs positively correlated with the lipid peroxidation activity. An increase in the content of DHNs was observed in cultures that differed in origin, growth indicators, and biochemical parameters, indicating the universality of this reaction. Thus, previously undescribed DHNs were identified, the accumulation of which is caused by water deficiency and is associated with manifestations of oxidative stress in the kidney cells of Scots pine.
在水分亏缺条件下，被称为脱水素（DHNs）的保护蛋白除了发挥抗氧化作用外，还能防止蛋白质和膜结构中的非特异性相互作用及其损伤。广泛分布的旱生植物欧洲赤松（Pinus sylvestris L.）的脱水素研究较少，其在抵抗水分亏缺中的作用尚未明确。在本文中，我们扩充了水分亏缺条件下欧洲赤松细胞中积累的脱水素列表，并揭示了它们与水分亏缺效应的关系。本研究采用欧洲赤松枝条和芽的愈伤组织培养物。通过向培养基中添加聚乙二醇制造轻度水分亏缺。在水分亏缺条件下，愈伤组织培养物中过氧化氢酶和过氧化物酶的活性增加。总含水量的适度降低与愈伤组织培养物生长速率的降低以及脂质过氧化活性的增加相关。Mr 72、38和27 kDa的脱水素在芽的愈伤组织培养物中积累，Mr 72和27 kDa脱水素的积累与脂质过氧化活性呈正相关。在起源、生长指标和生化参数不同的培养物中均观察到脱水素含量增加，表明该反应具有普遍性。因此，鉴定出了先前未描述的脱水素，其积累是由水分缺乏引起的，并且与欧洲赤松肾细胞中的氧化应激表现相关。
