Wu Yuanli, Mehdi Faisal, Cao Zhengying, Gan Yimei, Jiang Shuting, Zan Limei, Zhang Shuzhen, Yang Benpeng
National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China.
Plants (Basel). 2024 Oct 2;13(19):2767. doi: 10.3390/plants13192767.
To replace explosive nitrate-based chemicals in MS medium, this study developed a new, safer, and more cost-effective method using fertilizer-grade calcium ammonium nitrate and ammonium sulfate. This approach replaces ammonium nitrate and potassium nitrate, ensuring both safety and cost efficiency for sugarcane propagation. Six local sugarcane varieties-Zhongtang1 (ZT1), Zhongtang3 (ZT3), Zhongtang6 (ZT6), Guitang42 (GT42), Guitang44 (GT44), and Guiliu 07150 (GT07150)-were used. In the control group (Ck), nitrate ions (NO) were 39.28 mM, and ammonium ions (NH) were 20.49 mM, with a 2:1 ratio. In the treatment groups, the concentrations of nitrate ions (NO) and ammonium ions (NH) included treatment 1 (19.69 mM NO and 10.3 mM NH), treatment 2 (29.54 mM and 15.44 mM), treatment 3 (39.38 mM and 20.59 mM), treatment 4 (49.225 mM and 25.74 mM), treatment 5 (59.07 mM and 30.89 mM), and treatment 6 (68.915 mM and 36.03 mM), respectively, all with the same 2:1 ratio. Fifty bottles per treatment, with three replicates, were used for each sugarcane plantlets treatment. After five subcultures, the optimal ratio was determined by assessing morphological and physiological parameters, nitrogen levels, and SOD enzyme activity. The results indicated that treatment 3 (39.38 mM and 20.59 mM) and treatment 4 (49.225 mM and 25.74 mM) had the best morphological and physiological indicators. The optimal doses of calcium ammonium nitrate and ammonium sulfate were found in treatments 3 and 4, as well as in the control, with no significant difference among them. However, treatment 3, due to its lower dose, was more cost effective. To improve cost efficiency in practical production, it is recommended to use the lower concentration ratio of treatment 3 for plant tissue culture plantlets.
为了替代MS培养基中易爆的硝酸盐类化学物质，本研究开发了一种新的、更安全且更具成本效益的方法，该方法使用肥料级硝酸钙铵和硫酸铵。这种方法替代了硝酸铵和硝酸钾，确保了甘蔗繁殖的安全性和成本效益。使用了六个当地甘蔗品种——中糖1号（ZT1）、中糖3号（ZT3）、中糖6号（ZT6）、桂糖42号（GT42）、桂糖44号（GT44）和桂柳07150（GT07150）。在对照组（Ck）中，硝酸根离子（NO）为39.28 mM，铵根离子（NH）为20.49 mM，比例为2:1。在处理组中，硝酸根离子（NO）和铵根离子（NH）的浓度分别为处理1（19.69 mM NO和10.3 mM NH）、处理2（29.54 mM和15.44 mM）、处理3（39.38 mM和20.59 mM）、处理4（49.225 mM和25.74 mM）、处理5（59.07 mM和30.89 mM）以及处理6（68.915 mM和36.03 mM），所有处理的比例均为相同的2:1。每个甘蔗幼苗处理使用50瓶，每个处理重复三次。经过五次继代培养后，通过评估形态和生理参数、氮水平以及超氧化物歧化酶（SOD）活性来确定最佳比例。结果表明，处理3（39.38 mM和20.59 mM）和处理4（49.225 mM和25.74 mM）具有最佳的形态和生理指标。在处理3、处理4以及对照组中均发现了硝酸钙铵和硫酸铵的最佳剂量，它们之间没有显著差异。然而，处理3由于剂量较低，更具成本效益。为了在实际生产中提高成本效益，建议在植物组织培养幼苗中使用处理3的较低浓度比例。
