Xiao Qi, Qu Meng, Xie Jianping, Zhu Cigen, Shan Yuping, Mao Aihua, Qian Wenxian, Zhu Jiaping, Guo Jiahui, Lang Dong, Niu Jiaqiang, Wen Libin, He Kongwang
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China.
Animals (Basel). 2024 Oct 7;14(19):2882. doi: 10.3390/ani14192882.
Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. In this study, 2675 samples from 804 pig farms in 13 cities in Jiangsu Province, China, were collected between 2014 and 2021 and subjected to polymerase chain reaction analysis to investigate the frequency and genetic diversity of PCV2. The results showed that 41.42% (1108/2675) of samples tested positive for PCV2. The researchers further analyzed the genetic characteristics of 251 PCV2 strains and found that they belonged to the following four genotypes: PCV2a, PCV2b, PCV2d, and PCV2i. The dominant genotype was PCV2d, with a frequency of 49.80% (125/251). The detection rate of PCV2b was significantly higher than those of PCV2a and PCV2i, at 35.46% (89/251), 7.57% (19/251), and 7.17% (18/251), respectively. The percentage of different genotypes of PCV2 varied irregularly over time. We have further revealed the fingerprint of PCV2i genomic nucleotides for the first time. In conclusion, this study illustrates the high frequency and evolutionary features of PCV2 in Jiangsu Province over the past few years.
由猪圆环病毒2型（PCV2）引起的猪圆环病毒相关疾病广泛传播，给全球养猪业造成了重大经济损失。PCV2目前可分为9种基因型（PCV2a至PCV2i），当前占主导地位的是PCV2d基因型。在本研究中，于2014年至2021年期间收集了中国江苏省13个城市804个猪场的2675份样本，并进行聚合酶链反应分析，以调查PCV2的感染频率和遗传多样性。结果显示，41.42%（1108/2675）的样本PCV2检测呈阳性。研究人员进一步分析了251株PCV2毒株的遗传特征，发现它们属于以下四种基因型：PCV2a、PCV2b、PCV2d和PCV2i。优势基因型为PCV2d，频率为49.80%（125/251）。PCV2b的检出率显著高于PCV2a和PCV2i，分别为35.46%（89/251）、7.57%（19/251）和7.17%（18/251）。PCV2不同基因型的百分比随时间不规则变化。我们首次进一步揭示了PCV2i基因组核苷酸的指纹图谱。总之，本研究阐明了过去几年江苏省PCV2的高感染频率和进化特征。
