2016 - 2021年粤北地区猪圆环病毒2型的流行情况及遗传多样性

Prevalence and genetic diversity of porcine circovirus type 2 in northern Guangdong Province during 2016-2021.

作者信息

Nan Wenjin, Wu Jingbo, Hu Honghui, Peng Guoliang, Tan Simin, Deng Zhibang

机构信息

Lab of Animal Disease Prevention & Control and Animal Model, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), Changsha, China.

North Guangdong Collaborative Innovation and Development Center of Pig Farming and Disease Control, Shaoguan University, Shaoguan, China.

出版信息

Front Vet Sci. 2022 Aug 10;9:932612. doi: 10.3389/fvets.2022.932612. eCollection 2022.

DOI:10.3389/fvets.2022.932612

PMID:36032297

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9399655/

Abstract

The emergence and widespread of porcine circovirus-associated diseases (PCVADs), mainly caused by porcine circovirus type 2 (PCV2), threatens the Chinese swine industry. In this study, to investigate the recent prevalence of PCV2 in northern Guangdong Province of China, 573 tissue samples from 132 pig farms were collected during 2016-2021 and analyzed PCR. Overall, 51.38% (297/573, 95%CI 47.74-55.92) samples were tested PCV2 positive. The detection rate of PCV2 was significantly lower in samples collected before 2016-2018 than after the outbreak of African Swine Fever (2019-2021), being 59.85% (158/264, 95%CI 53.94-65.76) and 41.47% (141/340, 95%CI 36.43-46.71), respectively. On the other end, the genetic characteristics of 26 PCV2 strains were further analyzed. These PCV2 strains belonged to three genotypes, including PCV2a, PCV2b, and PCV2d. Specifically, the predominant genotype prevalent during two periods (2016-2018 and 2019-2021) wasPCV2b (81.82%, 9/11) and PCV2d (80.0%, 12/15), respectively. The results above illustrated the high prevalence and the genetic evolution feature of PCV2 in Guangdong Province in recent years.

摘要

猪圆环病毒相关疾病（PCVADs）的出现和广泛传播，主要由2型猪圆环病毒（PCV2）引起，对中国养猪业构成威胁。在本研究中，为调查中国广东省北部地区PCV2的近期流行情况，于2016 - 2021年期间从132个猪场收集了573份组织样本，并进行PCR分析。总体而言，51.38%（297/573，95%CI 47.74 - 55.92）的样本检测为PCV2阳性。2016 - 2018年之前收集的样本中PCV2的检出率显著低于非洲猪瘟爆发后（2019 - 2021年），分别为59.85%（158/264，95%CI 53.94 - 65.76）和41.47%（141/340，95%CI 36.43 - 46.71）。另一方面，对26株PCV2毒株的遗传特征进行了进一步分析。这些PCV2毒株属于三种基因型，包括PCV2a、PCV2b和PCV2d。具体而言，在两个时期（2016 - 2018年和2019 - 2021年）流行的主要基因型分别为PCV2b（81.82%，9/11）和PCV2d（80.0%，12/15）。上述结果说明了近年来广东省PCV2的高流行率和遗传进化特征。