正常猪血清对猪痢疾密螺旋体的杀菌作用。
Bactericidal effect of normal swine sera on Treponema hyodysenteriae.
作者信息
Joens L A, Nuessen M E
出版信息
Infect Immun. 1986 Jan;51(1):282-5. doi: 10.1128/iai.51.1.282-285.1986.
Treponema hyodysenteriae was incubated in 20% normal swine sera (NSS) at 37 degrees C for 4 h, and viability was determined by a plate dilution method. NSS was bactericidal for nonpathogenic T. innocens and avirulent T. hyodysenteriae, but not for virulent T. hyodysenteriae isolates. Heat inactivation at 56 degrees C for 30 min, treatment with EDTA or EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid], or removal of immunoglobulin M eliminated the bactericidal activity of NSS. However, removal of the alternate complement pathway with 10 mg of bentonite per ml did not remove bactericidal activity of NSS. Incubation of virulent isolates of T. hyodysenteriae in the presence of specific antisera plus NSS resulted in bactericidal activity. These data suggest that complement and natural antibody may be involved in protecting the host from T. innocens or avirulent T. hyodysenteriae and that T. hyodysenteriae antibody plus complement are involved in protecting convalescent pigs from re-exposure to swine dysentery.
将猪痢疾密螺旋体在37℃下于20%正常猪血清（NSS）中孵育4小时，并用平板稀释法测定其活力。NSS对非致病性无害密螺旋体和无毒力的猪痢疾密螺旋体具有杀菌作用，但对有毒力的猪痢疾密螺旋体分离株无杀菌作用。56℃加热灭活30分钟、用乙二胺四乙酸（EDTA）或乙二醇双（β-氨基乙基醚）-N,N-四乙酸（EGTA）处理或去除免疫球蛋白M均可消除NSS的杀菌活性。然而，每毫升加入10毫克膨润土去除替代补体途径并不能消除NSS的杀菌活性。在特异性抗血清和NSS存在的情况下孵育有毒力的猪痢疾密螺旋体分离株会产生杀菌活性。这些数据表明，补体和天然抗体可能参与保护宿主免受无害密螺旋体或无毒力猪痢疾密螺旋体的侵害，并且猪痢疾密螺旋体抗体加补体参与保护康复猪免受再次感染猪痢疾的侵害。
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