松花粉多糖和硫酸化多糖通过调节Th17/Treg对小鼠溃疡性结肠炎的影响

Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis in Mice by Regulating Th17/Treg.

作者信息

Wang Zhanjiang, Li Zhenxiang, Wang Hanyue, Wu Qiu, Geng Yue

机构信息

Key Laboratory of Food Nutrition and Safety of SDNU, College of Life Science, Shandong Normal University, Jinan 250358, China.

出版信息

Foods. 2024 Oct 7;13(19):3183. doi: 10.3390/foods13193183.

This study was to investigate the effects of the polysaccharides (PPM60-III) and sulfated polysaccharides (SPPM60-III) of pine pollen on the Th17/Treg balance, inflammatory cytokines, intestinal microbiota, and metabolite distribution in 3% DSS drinking water-induced UC mice. First of all, the physiological results showed that PPM60-III and SPPM60-III could alleviate UC, which was shown by the reduction in liver Treg cells, the rebalance of Th17/Treg, and the modulation of inflammatory cytokines. In addition, the 16S rRNA results showed that PPM60-III and SPPM60-III could decrease and , and increase , , and . Finally, the metabonomics results showed that PPM60-III and SPPM60-III also restored purine and glycerolipid metabolism, up-regulated nicotinate and nicotinamide metabolism and caffeine metabolism to inhibit inflammation. In conclusion, PPM60-III and SPPM60-III could inhibit UC by regulating gut bacteria composition and metabolite distribution; SPPM60-III showed better anti-colitis activity.

本研究旨在探讨松花粉多糖（PPM60 - III）和硫酸化多糖（SPPM60 - III）对3%葡聚糖硫酸钠（DSS）饮用水诱导的溃疡性结肠炎（UC）小鼠Th17/Treg平衡、炎性细胞因子、肠道微生物群及代谢物分布的影响。首先，生理结果表明，PPM60 - III和SPPM60 - III可缓解UC，表现为肝脏Treg细胞减少、Th17/Treg重新平衡以及炎性细胞因子的调节。此外，16S rRNA结果显示，PPM60 - III和SPPM60 - III可降低以及，并增加、以及。最后，代谢组学结果表明，PPM60 - III和SPPM60 - III还恢复了嘌呤和甘油脂质代谢，上调了烟酸和烟酰胺代谢以及咖啡因代谢以抑制炎症。总之，PPM60 - III和SPPM60 - III可通过调节肠道细菌组成和代谢物分布来抑制UC；SPPM60 - III表现出更好的抗结肠炎活性。