Niu Xiangyun, Shang Hongqi, Chen Siyan, Chen Ruichang, Huang Jin, Miao Yongqiang, Cui Wenping, Wang Huan, Sha Zhou, Peng Duo, Zhu Ruiliang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.
Food Funct. 2021 Jan 7;12(1):252-266. doi: 10.1039/d0fo02190c. Epub 2020 Dec 9.
The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine. On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology. Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation. However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown. In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice. The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics. Subsequently, we established immunosuppressive mice using cyclophosphamide (CTX) and found that PPPS regulated the immunosuppressive state of lymphocytes in Peyer's patches (PPs). Moreover, PPPS also regulated systemic immunity by acting on intestinal PPs. PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells. In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice. Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.
肠道微环境的稳定性是维持肠道正常生理活动的基础。相反，动态过程紊乱会导致慢性炎症和疾病病理。从泰山马尾松花粉中分离得到的马尾松花粉多糖（PPPS）已被报道具有广泛的生物活性，包括免疫调节作用。然而，PPPS在肠道微环境和肠道疾病中的作用仍不清楚。在这项工作中，我们通过使用16S rRNA高通量测序技术来评估PPPS对小鼠肠道微生物群的影响，从而展开我们的研究。结果表明，PPPS调节了小鼠肠道微生物群的组成，并增加了益生菌的比例。随后，我们使用环磷酰胺（CTX）建立了免疫抑制小鼠模型，发现PPPS调节了派尔集合淋巴结（PPs）中淋巴细胞的免疫抑制状态。此外，PPPS还通过作用于肠道PPs来调节全身免疫。PPPS减轻了脂多糖（LPS）诱导的Caco2细胞损伤，表明PPPS具有减少损伤并有效改善Caco2细胞屏障功能障碍的能力。此外，PPPS减轻了葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的结肠损伤并缓解了结肠炎症状。总体而言，我们的研究结果表明，PPPS在肠道微环境中显示出实际的调节作用，这为我们进一步开发PPPS的潜在应用价值提供了重要的理论依据。
