肠杆菌内质粒介导的柠檬酸盐利用的DNA序列保守性

Hirato T, Ishiguro N, Shinagawa M, Sato G

J Bacteriol. 1986 Jan;165(1):324-7. doi: 10.1128/jb.165.1.324-327.1986.

Southern blot DNA-DNA hybridization experiments with a cloned Cit+ DNA fragment as a probe showed that the plasmid-mediated Cit+ determinants from four Cit plasmids (R726, pOH3001, pOH3035, and pOH30221) were all homologous. Sequences homologous to the plasmid-borne Cit+ gene were also found in total bacterial DNA isolated from Salmonella paratyphi B, Salmonella enteritidis, Salmonella typhimurium LT-2, Citrobacter freundii, ATCC 8090, Citrobacter amalonaticus ATCC 25405, Klebsiella pneumoniae I and IID 977, and Enterobacter aerogenes ATCC 13048. The DNA digest from C. amalonaticus ATCC 25405 contained a 1.4-kilobase BamHI-HincII DNA fragment that was strongly homologous with and identical in size to the plasmid Cit+ probe.

以克隆的Cit⁺ DNA片段为探针进行的Southern印迹DNA-DNA杂交实验表明，来自四个Cit质粒（R726、pOH3001、pOH3035和pOH30221）的质粒介导的Cit⁺ 决定簇均具有同源性。在从副伤寒沙门氏菌B、肠炎沙门氏菌、鼠伤寒沙门氏菌LT-2、弗氏柠檬酸杆菌ATCC 8090、丙二酸柠檬酸杆菌ATCC 25405、肺炎克雷伯菌I和IID 977以及产气肠杆菌ATCC 13048中分离得到的总细菌DNA中也发现了与质粒携带的Cit⁺ 基因同源的序列。丙二酸柠檬酸杆菌ATCC 25405的DNA消化产物包含一个1.4千碱基的BamHI-HincII DNA片段，该片段与质粒Cit⁺ 探针高度同源且大小相同。