Siebor Eliane, de Curraize Claire, Amoureux Lucie, Neuwirth Catherine
Laboratory of Bacteriology, University Hospital of Dijon, Plateau technique de Biologie, BP 37013, 21070 Dijon cedex, France.
Laboratory of Bacteriology, University Hospital of Dijon, Plateau technique de Biologie, BP 37013, 21070 Dijon cedex, France
J Antimicrob Chemother. 2016 Aug;71(8):2167-70. doi: 10.1093/jac/dkw151. Epub 2016 May 5.
The objective of this study was to transfer the Salmonella genomic islands (GIs) SGI1 and SGI1-V and the Proteus GI PGI1-PmESC to clinical isolates of Enterobacteriaceae harbouring an A/C2 plasmid.
The entire genetic structures of SGI1 and PGI1-PmESC from Salmonella Typhimurium and Proteus mirabilis, respectively, were characterized by PCR and DNA sequencing. Ten enterobacterial isolates from different species carrying blaTEM-24 on an A/C2 plasmid were used for the mobilization of SGI1: Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter aerogenes, Citrobacter freundii, Klebsiella oxytoca, Proteus vulgaris, Providencia stuartii and Serratia marcescens. SGI1-V and PGI1-PmESC were transferred to E. aerogenes. Conjugation attempts were also performed using the wild strain E. aerogenes BOL and E. coli K-12 with or without pA/C2. Detection and location of the GI in the transconjugants were assessed by PCR targeting their junctions.
The multidrug resistance region of PGI1-PmESC contained a class 1 integron (aadB and aadA2) and regions deriving from transposon Tn501 and a hybrid Tn502/Tn5053 transposon, whereas SGI1 harboured the known determinants responsible for the pentaresistance. The transfer of SGI1 occurred from Salmonella Typhimurium to the 10 enterobacterial isolates, and transfer of SGI1-V and PGI1-PmESC occurred from P. mirabilis to E. aerogenes. In all transconjugants the GI was located at the 3'-end of trmE. SGI1 was also transferred to E. aerogenes BOL (pA/C2) and E. coli K-12 (pA/C2), but not to E. aerogenes BOL and E. coli K-12.
This is the first known description of SGI1 mobilization into a broad range of enterobacterial species harbouring an A/C2 plasmid and the first demonstration of PGI1 movement. The A/C2 plasmid is responsible for the GI mobilization.
本研究的目的是将沙门氏菌基因组岛（GI）SGI1和SGI1-V以及变形杆菌GI PGI1-PmESC转移至携带A/C2质粒的肠杆菌科临床分离株中。
分别通过PCR和DNA测序对鼠伤寒沙门氏菌的SGI1和奇异变形杆菌的PGI1-PmESC的完整遗传结构进行了表征。使用10株在A/C2质粒上携带blaTEM-24的不同种肠杆菌分离株来进行SGI1的转移：大肠杆菌、阴沟肠杆菌、肺炎克雷伯菌、奇异变形杆菌、产气肠杆菌、弗氏柠檬酸杆菌、产酸克雷伯菌、普通变形杆菌、斯氏普罗威登斯菌和粘质沙雷氏菌。将SGI1-V和PGI1-PmESC转移至产气肠杆菌。还使用野生型产气肠杆菌BOL和大肠杆菌K-12（有无pA/C2）进行了接合试验。通过针对其连接点的PCR评估转接合子中GI的检测和定位。
PGI1-PmESC的多药耐药区域包含1类整合子（aadB和aadA2）以及源自转座子Tn501和杂交Tn502/Tn5053转座子的区域，而SGI1含有导致五重耐药的已知决定簇。SGI1从鼠伤寒沙门氏菌转移至10株肠杆菌分离株，SGI1-V和PGI1-PmESC从奇异变形杆菌转移至产气肠杆菌。在所有转接合子中，GI位于trmE的3'端。SGI1也转移至产气肠杆菌BOL（pA/C2）和大肠杆菌K-12（pA/C2），但未转移至产气肠杆菌BOL和大肠杆菌K-12。
这是关于SGI1转移至携带A/C2质粒的多种肠杆菌科物种的首次已知描述，也是PGI1移动的首次证明。A/C2质粒负责GI的转移。
