Department of Pharmaceutical Sciences, Faculty of Pharmacy, AlMaarefa University, Diriyah, Riyadh 13713, Saudi Arabia.
Department of Pharmacognosy and Medicinal Herbs, Faculty of Pharmacy, Al-Baha University, Al Baha, Saudi Arabia.
Diagn Microbiol Infect Dis. 2024 Dec;110(4):116558. doi: 10.1016/j.diagmicrobio.2024.116558. Epub 2024 Oct 12.
This study aimed to evaluate the antimicrobial susceptibility and combination of a beta-blocker, labetalol (LAB) and meropenem (MEM) on Carbapenem-resistant (CR) A. baumannii clinical isolates. A total of 43 CR- A. baumannii were isolated of which 37 (86.6 %) and 28 (65 %) exhibited MDR and XDR phenotypes, respectively. Colistin and doxycycline still retain their activities in 93.1 % and 72.1 % of the isolates, respectively. Combining MEM with LAB at 0.25 mg /mL, decreased MIC values in 91.4 % (32/35) however, at 0.5 mg /mL, it decreased MIC value and restored susceptibility to MEM in 100 % and 91.4 % of the tested isolates, respectively. A novel transferable plasmid pAcbGIM3 harboring aph-3', blabla and bla and eight mobile genetic elements were successfully isolated from a pan-drug resistant (PDR) isolate. In conclusion, LAB-MEM is a promising combination and should be clinically examined. This is the first report of a transmissible plasmid harboring bla gene in Egypt.
本研究旨在评估β受体阻滞剂拉贝洛尔（LAB）与美罗培南（MEM）联合应用对耐碳青霉烯鲍曼不动杆菌（CR-AB）临床分离株的抗菌药敏情况。共分离出 43 株 CR-AB，其中 37 株（86.6%）和 28 株（65%）分别表现出多药耐药和广泛耐药表型。多粘菌素和强力霉素对 93.1%和 72.1%的分离株仍保持活性。MEM 与 LAB 以 0.25mg/ml 联合使用时，可降低 91.4%（32/35）的 MIC 值，但以 0.5mg/ml 联合使用时，可降低 MIC 值并使 MEM 对 100%和 91.4%的受试分离株恢复敏感性。从一株泛耐药（PDR）分离株中成功分离出携带 aph-3'、bla bla 和 bla 基因及 8 种移动遗传元件的新型可转移质粒 pAcbGIM3。综上所述，LAB-MEM 是一种有前景的联合用药，值得临床进一步研究。这是首次在埃及报道携带 bla 基因的可传播质粒。
