MvaT negatively regulates pyocin S5 expression in .

Regulatory mechanisms that direct the synthesis and release of pyocin S5, a surface-acting bacteriocin produced by , are relatively unknown. This study aims to identify transcription factors that regulate pyocin S5 expression in PAO1. We captured the transcription factor MvaT using the promoter region upstream of gene (S5P). Further, we demonstrated specific binding of MvaT and its paralog MvaU to S5P using a gel-shift assay. Lastly, we showed that MvaT negatively regulates the gene expression by gene deletion and transcriptomic analysis. Our findings provide valuable insights into the regulation of pyocin S5 production, which paves the way to develop novel therapeutics against infections.