Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
Department of Orthopaedic Surgery, Herlev Hospital, Herlev, Denmark.
BMJ Open. 2024 Oct 17;14(10):e084081. doi: 10.1136/bmjopen-2024-084081.
Chronic wounds affect 1%-2% of the global population, with rising incidence due to ageing and lifestyle-related diseases. Bacterial biofilms, found in 80% of chronic wounds, and scattered single-cell bacteria may hinder healing. Microbes are believed to negatively impact healing by exacerbating inflammation and host immune response.
The primary objective of the chronic wound characterisation (CWC) study is to investigate chronic wounds through a prospective observational cohort study exploring bacterial community composition, inflammatory responses and the influence of bacteria on wound-healing trajectories. The CWC study will be investigated through two cohorts: the and .The includes patients with a chronic wound scheduled for mechanical debridement. The debrided material will be collected for dual RNA sequencing and 16s ribosomal RNA gene sequencing, as well as samples for microbial culturing and a photo to assess the wound. Clinical data is recorded, and healing and/or other clinical endpoints are established through medical records.The includes and follows patients undergoing split-thickness skin grafting. Extensive sampling (ESwabs, biopsies, tape strips, debrided material and a sample of the skin graft) will be performed on surgery and patients will be seen at two follow-up visits. Samples will be analysed through culturing and next-generation sequencing methods. A biobank will be established comprising longitudinal clinical samples and clinical data.
The study has been approved by the board of health ethics, Capital Region of Denmark, under protocol number H-20032214. The study findings will be disseminated through peer-reviewed publications and showcased at both national and international conferences and meetings within the domains of microbiology, wound healing and infection.
慢性伤口影响全球 1%-2%的人口，由于人口老龄化和与生活方式相关的疾病，发病率不断上升。在 80%的慢性伤口中发现的细菌生物膜和分散的单细胞细菌可能会阻碍愈合。微生物被认为通过加剧炎症和宿主免疫反应对愈合产生负面影响。
慢性伤口特征（CWC）研究的主要目的是通过前瞻性观察队列研究探索细菌群落组成、炎症反应以及细菌对伤口愈合轨迹的影响来研究慢性伤口。CWC 研究将通过两个队列进行调查： 和 。 包括计划进行机械清创的慢性伤口患者。将收集清创材料进行双 RNA 测序和 16s 核糖体 RNA 基因测序，以及用于微生物培养和评估伤口的照片的样本。记录临床数据，并通过病历确定愈合和/或其他临床终点。 包括并随访接受断层皮片移植的患者。将在手术时对患者进行广泛采样（ESwabs、活检、胶带条、清创材料和皮肤移植物样本），并在两次随访就诊时进行。将通过培养和下一代测序方法分析样本。将建立一个生物银行，其中包含纵向临床样本和临床数据。
该研究已获得丹麦首都大区卫生局伦理委员会的批准，编号为 H-20032214。研究结果将通过同行评审的出版物传播，并在微生物学、伤口愈合和感染领域的国内外会议和会议上展示。
