新型菲律宾蛭素提取物对 HeLa 细胞中 EGFR 基因及其蛋白表达的分子调控作用。

Effect control of novel Hirudinaria manillensis extract on the molecular regulation of EGFR gene and its protein expression in HeLa cells.

机构信息

PG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.

Department of Zoology, Thanthai Periyar Government Arts and Science College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620023, India.

出版信息

Mol Biol Rep. 2024 Oct 17;51(1):1061. doi: 10.1007/s11033-024-10016-7.

DOI:10.1007/s11033-024-10016-7

PMID:39419922

Abstract

BACKGROUND

Most anticancer drugs possess the capacity to control precise molecular processes and gene-regulated protein expressions, which could enhance the efficacy of cancer treatments. Hirudinaria manillensis is prevalent in South Asia and has been employed for several decades in medical conditions based on its curative benefits.

METHODS AND RESULTS

The present study aimed to explore the molecular regulation of the target EGFR gene, and the protein expression of EGFR on the HeLa cell line. To achieve our aim, quantitative real-time PCR and immunocytochemistry assays were conducted. Interestingly, qRT-PCR results confirmed the downregulation of the EGFR gene on the HeLa cells in comparison with the β- actin internal control gene. Furthermore, immunocytochemistry assay results confirmed the decreasing level of EGFR gene expression in the HeLa cells.

CONCLUSION

Therefore, Hirudinaria manillensis could be a promising anticancer candidate for cervical cancer treatment.

摘要

背景

大多数抗癌药物具有控制精确分子过程和基因调控蛋白表达的能力，这可以提高癌症治疗的效果。菲律宾医蛭在南亚很常见，几十年来，由于其治疗功效，已被用于多种医疗条件。

方法和结果

本研究旨在探索 EGFR 基因的分子调控以及 HeLa 细胞系中 EGFR 的蛋白表达。为了达到我们的目的，我们进行了定量实时 PCR 和免疫细胞化学分析。有趣的是，qRT-PCR 结果证实与β-肌动蛋白内参基因相比，EGFR 基因在 HeLa 细胞中下调。此外，免疫细胞化学分析结果证实 HeLa 细胞中 EGFR 基因表达水平降低。