Knight S C, Balfour B, O'Brien J, Buttifant L
Transplantation. 1986 Jan;41(1):96-100. doi: 10.1097/00007890-198601000-00019.
The effect of cyclosporine (CsA) on dendritic cell function was tested by pulsing rabbit veiled cells (VC, the "dendritic" cells from the afferent lymph) with the drug. This treatment inhibited their capacity to enhance proliferative responses of autologous peripheral blood lymphocytes to the mitogen concanavalin A. The concentration of CsA required to produce inhibition varied from one rabbit to another and ranged from 5 to 100 ng/ml. The degree of inhibition was greater under conditions in which the normal VC were more effective. Addition of further untreated VC restored the response, but this restoration was incomplete under some culture conditions. Pulsing peripheral blood mononuclear cells (PBM) with CsA also produced a reduction in the stimulation by Con A, and the response was not restored by addition of normal VC. The experiments did not distinguish whether the effect of CsA was directly on dendritic cell function or via a secondary effect of "presentation" of CsA to lymphocytes by the dendritic cells.
通过用环孢素（CsA）处理兔面纱细胞（VC，即来自输入淋巴管的“树突状”细胞）来测试其对树突状细胞功能的影响。这种处理抑制了它们增强自体外周血淋巴细胞对有丝分裂原刀豆球蛋白A增殖反应的能力。产生抑制所需的CsA浓度因兔子而异，范围为5至100 ng/ml。在正常VC更有效的条件下，抑制程度更大。添加未处理的VC可恢复反应，但在某些培养条件下这种恢复并不完全。用CsA处理外周血单个核细胞（PBM）也会导致刀豆球蛋白A刺激的降低，并且添加正常VC不能恢复反应。这些实验没有区分CsA的作用是直接作用于树突状细胞功能，还是通过树突状细胞将CsA“呈递”给淋巴细胞的继发效应。
