Knight S C, Balfour B M, O'Brien J, Buttifant L, Sumerska T, Clarke J
Eur J Immunol. 1982 Dec;12(12):1057-60. doi: 10.1002/eji.1830121214.
Peripheral blood lymphocytes from normal rabbits or from rabbits hyperimmunized with human immunoglobulin were cultured in 20-microliters hanging droplets. The cultures were stimulated with concanavalin A or, in the case of cells from sensitized animals, with human immunoglobulin. The addition to the cultures of small numbers of autologous or allogeneic veiled cells separated from afferent lymph increased the responses to low doses of the stimulants, particularly when the cells were cultured at low cell densities or for short periods of time. At high cell densities or at longer periods of culture, stimulation occurred in the absence of added veiled cells and was associated with clumping of the lymphoid cells and development of cells which morphologically resembled veiled cells found in afferent lymph. The enhanced responses upon addition of small numbers of veiled cells were also correlated with the formation of lymphoid aggregates which were frequently held together by the elongated processes of a single veiled cell. The observations support the view that the veiled cells are lymph-borne precursors of dendritic cells in the lymph nodes. This may provide an in vitro model for the cellular relationships which occur in paracortical cords of lymph nodes following antigenic stimulation.
将正常兔或用人免疫球蛋白超免疫的兔的外周血淋巴细胞培养于20微升悬滴中。培养物用刀豆球蛋白A刺激，或者对于来自致敏动物的细胞，用人免疫球蛋白刺激。向培养物中添加从输入淋巴管分离的少量自体或同种异体隐蔽细胞，可增强对低剂量刺激物的反应，特别是当细胞以低细胞密度培养或培养较短时间时。在高细胞密度或较长培养时间时，在未添加隐蔽细胞的情况下也会发生刺激，这与淋巴细胞的聚集以及形态上类似于在输入淋巴管中发现的隐蔽细胞的细胞发育有关。添加少量隐蔽细胞后反应增强也与淋巴样聚集体的形成相关，这些聚集体经常由单个隐蔽细胞的细长突起聚集在一起。这些观察结果支持这样一种观点，即隐蔽细胞是淋巴结中树突状细胞的淋巴源性前体。这可能为抗原刺激后淋巴结副皮质索中发生的细胞关系提供一个体外模型。
