Simões Milena Luzorio, Brandão Felipe Zandonadi, Santos Juliana Dantas Rodrigues, Pinto Pedro Henrique Nicolau, Schmidt Ana Paula Pereira, Laeber Camila Correa Roza, Knust Nathália Dutra, Rodriguez Mariana Garcia Kako, Fila Danilo, Vázquez María Isabel, Ungerfeld Rodolfo
Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brasil.
Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Anim Reprod. 2024 Oct 7;21(4):e20240073. doi: 10.1590/1984-3143-AR2024-0073. eCollection 2024.
The study aimed to evaluate the effectiveness of combining two injectable progesterone (iP4) formulas for estrous synchronization in ewes and to compare it with traditional intravaginal progesterone devices. Additionally, the study assessed whether the inclusion of GnRH enhances the reproductive outcomes of the iP4 treatment. Two experiments were conducted. In the first experiment, 20 Santa Inês ewes were divided into two groups: one group received intravaginal progesterone devices, and the other received combined long-acting and short-acting iP4. In the second experiment, 30 Corriedale ewes were divided into two groups: one received the combined iP4 with GnRH, and the other without GnRH. Estrous, ovulation, follicular populations, and progesterone concentrations were monitored. The combined iP4 treatment induced an artificial luteal phase and produced reproductive responses similar to those obtained with intravaginal devices. In the first experiment, the iP4 treatment tended to result in more synchronized ovulation compared to the control (P=0.095). In the second experiment, adding GnRH enhanced the quality of the corpus luteum, as indicated by increased diameter and vascularization on Day 23 (P=0.047 and P=0.02, respectively). The combined administration of long-acting and short-acting iP4 effectively synchronized estrous in ewes and showed similar efficacy to traditional intravaginal devices. The inclusion of GnRH improved luteal quality, suggesting potential benefits for reproductive management in ewes. Further studies are needed to evaluate the fertility outcomes of these protocols under field conditions.
该研究旨在评估两种注射用孕酮（iP4）配方联合使用对母羊发情同步化的有效性，并将其与传统的阴道内孕酮装置进行比较。此外，该研究还评估了添加促性腺激素释放激素（GnRH）是否能提高iP4治疗的生殖效果。进行了两项实验。在第一个实验中，将20只圣伊内斯母羊分为两组：一组接受阴道内孕酮装置，另一组接受长效和短效iP4联合治疗。在第二个实验中，将30只考力代母羊分为两组：一组接受iP4与GnRH联合治疗，另一组不接受GnRH。监测发情、排卵、卵泡数量和孕酮浓度。联合iP4治疗诱导了人工黄体期，并产生了与阴道内装置相似的生殖反应。在第一个实验中，与对照组相比，iP4治疗倾向于导致更同步的排卵（P=0.095）。在第二个实验中，添加GnRH提高了黄体质量，在第23天黄体直径和血管化增加表明了这一点（分别为P=0.047和P=0.02）。长效和短效iP4联合给药有效地使母羊发情同步化，并显示出与传统阴道内装置相似的疗效。添加GnRH改善了黄体质量，表明对母羊生殖管理有潜在益处。需要进一步研究来评估这些方案在田间条件下的生育结果。
