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麦芽制造是绝对必要的吗?原生黑小麦在酿造过程中作为大麦麦芽的替代品。

Is malting an absolute must? Native triticale as a stand-in for barley malt in the brewing process.

作者信息

Pribić Milana, Mejić Luka, Despotović Saša, Špirović-Trifunović Bojana, Bulut Sandra, Pejin Jelena

机构信息

Department of Biotechnology, Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, Serbia.

Department of Computing and Control Engineering, Faculty of Technical Sciences, University of Novi Sad, Novi Sad, Serbia.

出版信息

J Sci Food Agric. 2025 Feb;105(3):1818-1829. doi: 10.1002/jsfa.13959. Epub 2024 Oct 18.

Abstract

BACKGROUND

To remain competitive, brewers must innovate by incorporating novel elements beyond traditional styles. Thus, exploring triticale as a modern substitute for barley malt is promising, especially given its higher amylolytic activity compared to barley. This study aimed to assess the impact of substituting up to 50% of barley malt with unmalted triticale on green beer quality, encompassing multiple stages from wort production to primary fermentation at a laboratory scale.

RESULTS

Triticale-based worts (ratios 10-50%) had lower extract content than 100% barley malt. However, incorporating 10% of triticale led to only a 1% decrease in extract content compared to the all-malt wort. Shearzyme® 500L, an endo-1,4-β-xylanase with β-glucanase side activity, effectively addressed wort viscosity by breaking down arabinoxylans and β-glucans in triticale cell walls. All triticale-based beers exhibited lower ethanol content compared to reference beer, as is typical when using adjuncts. In green beer, a 50% triticale ratio lowered ethanol content by 16% (without enzyme) and 19% (with enzyme) compared to 100% malt beer. However, green beer with 10% triticale had satisfactory levels of total polyphenol and vicinal diketone content, among other parameters.

CONCLUSION

Commercial enzyme application significantly enhanced proteolytic activity within the grain. Fermentations of enzyme-treated worts showed higher amino acid levels, further confirming the increased proteolytic activity facilitated by the chosen enzyme. Overall, this study provides a comprehensive analysis of the brewing process using native triticale. Building on this foundation, future studies will focus on optimizing mashing conditions to enhance the fermentation profile of the wort. © 2024 Society of Chemical Industry.

摘要

背景

为保持竞争力,啤酒酿造商必须通过融入传统风格之外的新元素进行创新。因此,探索用小黑麦作为大麦麦芽的现代替代品很有前景,特别是考虑到其与大麦相比具有更高的淀粉分解活性。本研究旨在评估用未发芽的小黑麦替代高达50%的大麦麦芽对嫩啤酒质量的影响,涵盖从麦芽汁生产到实验室规模的主发酵的多个阶段。

结果

以小黑麦为基础的麦芽汁(比例为10%-50%)的浸出物含量低于100%大麦麦芽。然而,与全麦芽麦芽汁相比,加入10%的小黑麦只会使浸出物含量降低1%。Shearzyme® 500L是一种具有β-葡聚糖酶侧活性的内切-1,4-β-木聚糖酶,通过分解小黑麦细胞壁中的阿拉伯木聚糖和β-葡聚糖有效地解决了麦芽汁粘度问题。与参考啤酒相比,所有以小黑麦为基础的啤酒的乙醇含量都较低,这在使用辅料时是很常见的。在嫩啤酒中,与100%麦芽啤酒相比,50%小黑麦比例使乙醇含量降低了16%(无酶)和19%(有酶)。然而,含有10%小黑麦的嫩啤酒在总多酚和连位二酮含量等其他参数方面具有令人满意的水平。

结论

商业酶的应用显著提高了谷物中的蛋白水解活性。酶处理麦芽汁的发酵显示出更高的氨基酸水平,进一步证实了所选酶促进了蛋白水解活性的提高。总体而言,本研究对使用天然小黑麦的酿造过程进行了全面分析。在此基础上,未来的研究将集中在优化糖化条件以改善麦芽汁的发酵特性。© 2024化学工业协会。

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