Ju Zijing, Cui Lulu, Lei Changwei, Song Mengze, Chen Xuan, Liao Ziwei, Zhang Tiejun, Wang Hongning
Laboratory of Bio-Resource and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610017, China.
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu 610064, China.
Antibiotics (Basel). 2023 Nov 19;12(11):1642. doi: 10.3390/antibiotics12111642.
Non-typhoidal salmonellosis is a dangerous foodborne disease that causes enormous economic loss and threatens public health worldwide. The consumption of food, especially poultry or poultry products, contaminated with non-typhoidal (NTS) is the main cause of human salmonellosis. To date, no research has identified the molecular epidemiological characteristics of NTS strains isolated from breeder chicken farms in different provinces of China. In our study, we investigated the antimicrobial resistance, phylogenetic relationships, presence of antimicrobial resistance and virulence genes, and plasmids of NTS isolates recovered from breeder chicken farms in five provinces of China between 2020 and 2021 by using a whole-genome sequencing (WGS) approach and phenotypic methods. All sequenced isolates belonged to six serovars with seven sequence types. Nearly half of the isolates (44.87%) showed phenotypic resistance to at least three classes of antimicrobials. serotype Kentucky harbored more antimicrobial resistance genes than the others, which was highly consistent with phenotypic resistance. Furthermore, the carried rate of 104 out of 135 detected virulence genes was 100%. Overall, our WGS results highlight the need for the continuous monitoring of, and additional studies on, the antimicrobial resistance of NTS.
非伤寒沙门氏菌病是一种危险的食源性疾病，会造成巨大的经济损失，并威胁全球公共卫生。食用被非伤寒沙门氏菌（NTS）污染的食物，尤其是家禽或家禽产品，是人类感染沙门氏菌病的主要原因。迄今为止，尚无研究确定从中国不同省份的种鸡场分离出的NTS菌株的分子流行病学特征。在我们的研究中，我们使用全基因组测序（WGS）方法和表型方法，调查了2020年至2021年间从中国五个省份的种鸡场分离出的NTS菌株的抗菌药物耐药性、系统发育关系、抗菌药物耐药基因和毒力基因的存在情况以及质粒。所有测序菌株属于六个血清型，有七种序列类型。近一半的菌株（44.87%）对至少三类抗菌药物表现出表型耐药性。肯塔基血清型携带的抗菌药物耐药基因比其他血清型更多，这与表型耐药性高度一致。此外，在检测到的135个毒力基因中，104个的携带率为100%。总体而言，我们的WGS结果凸显了持续监测NTS抗菌药物耐药性并开展更多研究的必要性。
