Department of Obstetrics and Gynecology, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, 213000, PR China.
Suqian First People's Hospital of Nanjing Medical University, Suqian, Jiangsu Province, 223600, PR China.
J Ovarian Res. 2024 Oct 18;17(1):206. doi: 10.1186/s13048-024-01531-z.
To establish a rat model of pharmacological ovariectomy by GnRH-a injection and to preliminarily investigate the reproductive endocrine effects of Xiangshao granules on pharmacologically ovariectomized rats.
A rat model of pharmacological ovariectomy was established by injecting female rats with Gonadotropin-releasing hormone agonist(GnRH-a).The rats were randomly divided into four groups: GnRH-a injected saline group (GnRH-a + NS); GnRH-a injected oestradiol group (GnRH-a + E2); GnRH-a injected Xiangshao granule group (GnRH-a + Xiangshao), and the control group of saline-injected rats (NS + NS). The number of rats per group was 6.According to observations of the rats' vaginal smears, modelling was determined as successful. Then corresponding drug gavage intervention was administered for 28 days, and rat body weight and anal temperature were measured every other day to adjust the drug intervention amount according to body weight changes. Plasma sex hormone levels (E2, FSH, LH), uterine weight, uterine index and endometrial histomorphological changes, ovarian weight, and ovarian index and ovarian histomorphological changes were measured in each group after the gavage.
(1) Plasma sex hormone levels (E2, FSH, LH) of the GnRH-a + NS, GnRH-a + E2, and GnRH-a + Xiangshao granule groups were significantly lower than the NS + NS group (P < 0.001), while the E2 level of the GnRH-a + E2 group was higher than that of the GnRH-a + Xiangshao granule group (P < 0.05). The FSH level of the GnRH-a + E2 group was significantly lower than that of the GnRH-a + Xiangshao granule group (P < 0.05). The LH level of the GnRH-a + E2 group was significantly lower than those in the GnRH-a + NS and GnRH-a + Xiangshao granule groups (P < 0.001, P = 0.001). The LH and FSH levels of the GnRH-a + NS and GnRH-a + Xiangshao granule groups were not significantly different (P > 0.05). (2) Compared with the NS + NS group, the uterine weight and uterine index, and ovarian weight and ovarian index of GnRH-a injected rats in each model all significantly decreased (P < 0.001). Between the groups, the uterine weight and uterine index, and ovarian weight and ovarian index of GnRH-a + E2 and GnRH-a + Xiangshao granule groups were all significantly higher than those of the GnRH-a + NS group (P < 0.001, P < 0.05). The uterine weight and uterine index, and ovarian weight and ovarian index of the GnRH-a + E2 group increased compared with the GnRH-a + Xiangshao granule group (P < 0.05). (3) Compared with the NS + NS group, the number of primordial follicles of the GnRH-a + NS, GnRH-a + E2, and GnRH-a + Xiangshao granule groups increased significantly and the number of growing follicles and mature follicles significantly decreased. (4) Rats' uterine wall of the NS + NS and various GnRH-a groups was significantly thinner, with the endothelial layer atrophied, while the uterine wall of the GnRH-a + E2 and GnRH-a + Xiangshao granule groups was thicker obviously, with the number of vaginal folds and blood vessels also increasing. Specifically, the uterus and vagina improvements in the GnRH-a + E2 group were more obvious than in GnRH-a + NS and GnRH-a + Xiangshao granule groups.
GnRH-a injection can reduce the levels of sex hormones E2, FSH, and LH in rats, causing perimenopausal symptoms such as hot flashes, while Xiangshao and E2 granules could significantly improve such symptoms and exert a slight oestrogenic effect, to a lesser extent than E2 does.
Not applicable.
通过 GnRH-a 注射建立大鼠药理学卵巢切除术模型，并初步探讨香芍颗粒对药理去卵巢大鼠生殖内分泌的影响。
通过给雌性大鼠注射促性腺激素释放激素激动剂（GnRH-a）建立药理学卵巢切除术大鼠模型。将大鼠随机分为 4 组：GnRH-a 生理盐水组（GnRH-a+NS）、GnRH-a 雌二醇组（GnRH-a+E2）、GnRH-a 香芍颗粒组（GnRH-a+Xiangshao）和生理盐水注射对照大鼠组（NS+NS）。每组大鼠 6 只。根据大鼠阴道涂片观察，确定模型成功。然后进行相应的药物灌胃干预 28 天，根据体重变化调整药物干预量，每隔一天测量大鼠体重和肛温。灌胃后测量各组大鼠血浆性激素水平（E2、FSH、LH）、子宫重量、子宫指数和子宫内膜组织形态学变化、卵巢重量、卵巢指数和卵巢组织形态学变化。
（1）GnRH-a+NS、GnRH-a+E2 和 GnRH-a+Xiangshao 颗粒组大鼠血浆性激素水平（E2、FSH、LH）明显低于 NS+NS 组（P<0.001），而 GnRH-a+E2 组 E2 水平高于 GnRH-a+Xiangshao 颗粒组（P<0.05）。GnRH-a+E2 组 FSH 水平明显低于 GnRH-a+Xiangshao 颗粒组（P<0.05）。GnRH-a+E2 组 LH 水平明显低于 GnRH-a+NS 和 GnRH-a+Xiangshao 颗粒组（P<0.001，P=0.001）。GnRH-a+NS 和 GnRH-a+Xiangshao 颗粒组 LH 和 FSH 水平无明显差异（P>0.05）。（2）与 NS+NS 组相比，各模型大鼠子宫重量和子宫指数、卵巢重量和卵巢指数均明显降低（P<0.001）。与 GnRH-a+NS 组相比，GnRH-a+E2 和 GnRH-a+Xiangshao 颗粒组大鼠子宫重量和子宫指数、卵巢重量和卵巢指数均明显升高（P<0.001，P<0.05）。GnRH-a+E2 组大鼠子宫重量和子宫指数、卵巢重量和卵巢指数均高于 GnRH-a+Xiangshao 颗粒组（P<0.05）。（3）与 NS+NS 组相比，GnRH-a+NS、GnRH-a+E2 和 GnRH-a+Xiangshao 颗粒组大鼠原始卵泡数明显增多，生长卵泡和成熟卵泡数明显减少。（4）NS+NS 和各 GnRH-a 组大鼠子宫壁明显变薄，内皮层萎缩，而 GnRH-a+E2 和 GnRH-a+Xiangshao 颗粒组大鼠子宫壁明显增厚，阴道褶皱和血管数量增加。具体来说，GnRH-a+E2 组的子宫和阴道改善比 GnRH-a+NS 和 GnRH-a+Xiangshao 颗粒组更明显。
GnRH-a 注射可降低大鼠体内性激素 E2、FSH 和 LH 水平，引起潮热等围绝经期症状，而香芍和雌二醇颗粒可明显改善这些症状，并发挥轻微的雌激素作用，作用程度小于雌二醇。
不适用。
