估算全动物体内酶促药物代谢的动力学参数。
Estimating the kinetic parameters for enzymatic drug metabolism in the whole animal.
作者信息
Watson J V, Workman P
出版信息
Biochem Pharmacol. 1986 Jan 15;35(2):145-9. doi: 10.1016/0006-2952(86)90507-1.
A method for estimating the Michaelis constant, Km, and the maximum reaction velocity, Vmax, for the enzymatic degradation of a parent compound to a metabolite in the intact animal is presented. The technique involves a mathematical analysis which has shown that under specific conditions the peak/plateau blood concentrations of metabolite are related to initial parent compound concentration by the Michaelis-Menten relationship. It has also been shown how these data can be analysed with the "direct linear plot" of Eisenthal and Cornish-Bowden (Biochem. J. 139, 715 (1974)) to yield the enzyme kinetic parameters.
本文提出了一种在完整动物体内估算母体化合物酶促降解为代谢物的米氏常数（Km）和最大反应速度（Vmax）的方法。该技术涉及数学分析，结果表明在特定条件下，代谢物的血药浓度峰值/平台期与母体化合物初始浓度符合米氏方程关系。同时还展示了如何使用艾森塔尔和康沃尔-鲍登的“直接线性作图法”（《生物化学杂志》139, 715 (1974)）对这些数据进行分析，以得出酶动力学参数。
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