通过直接线性作图法估计米氏动力学参数的改进非参数统计方法。
Improved non-parametric statistical methods for the estimation of Michaelis-Menten kinetic parameters by the direct linear plot.
作者信息
Porter W R, Trager W F
出版信息
Biochem J. 1977 Feb 1;161(2):293-302. doi: 10.1042/bj1610293.
Abstract
The theoretical basis for the direct linear plot [Eisenthal & Cornish-Bowden (1974) Biochem. J. 139, 715-720], a non-parametric statistical method for the analysis of data-fitting the Michaelis-Menten equation, was reinvestigated in order to accommodate additional experimental designs and to provide estimates of precision more directly comparable with those obtained by parametric statistical methods. Methods are given for calculating upper and lower confidence limits for the estimated parameters, for accommodating replicate measurements and for comparing the results of two separate experiments. Factors that influence the proper design of experiments are discussed.
摘要
直接线性作图法[艾斯enthal和康沃尔-鲍登（1974年），《生物化学杂志》139卷，715 - 720页]是一种用于分析符合米氏方程数据的非参数统计方法，为了适应更多的实验设计，并提供与参数统计方法获得的精度估计更直接可比的精度估计，我们对其理论基础进行了重新研究。文中给出了计算估计参数的上下置信限、处理重复测量以及比较两个独立实验结果的方法。还讨论了影响实验合理设计的因素。
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本文引用的文献
1
The statistical analysis of enzyme kinetic data.酶动力学数据的统计分析。
Adv Enzymol Relat Areas Mol Biol. 1967;29:1-32. doi: 10.1002/9780470122747.ch1.
2
Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods.通过直接线性作图法及其他方法估算酶动力学参数时的统计学考量
Biochem J. 1974 Jun;139(3):721-30. doi: 10.1042/bj1390721.
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An analysis of the depressor responses to histamine in the cat and dog: involvement of both H1- and H2-receptors.猫和狗对组胺降压反应的分析：H1和H2受体均参与其中。
Br J Pharmacol. 1975 Jul;54(3):319-24. doi: 10.1111/j.1476-5381.1975.tb07571.x.
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