Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China.
Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China.
J Biotechnol. 2024 Dec 10;396:28-35. doi: 10.1016/j.jbiotec.2024.10.008. Epub 2024 Oct 18.
To enhance the activity of Lactiplantibacillus plantarum FS4722 (L. plantarum FS4722) in reducing uric acid, this study optimized the culture medium components and fermentation conditions using response surface methodology. The results indicated that the optimal culture medium comprised glucose at 25.61 g/L, yeast extract powder (YEP) at 26.86 g/L, and nucleoside addition at 0.48 g/L. The optimal fermentation conditions were: a fermentation time of 10 h, an initial pH of 6.5, a fermentation temperature of 33 °C, and an inoculum size of 1.0 %. Under these conditions, the nucleoside degradation ability of L. plantarum FS4722 increased by 41.9 times, enhancing its potential applications in reducing uric acid and developing anti-gout foods, health supplements, and pharmaceuticals.
为了提高植物乳杆菌 FS4722（L. plantarum FS4722）降低尿酸的活性，本研究采用响应面法优化了培养基成分和发酵条件。结果表明，最佳培养基组成为葡萄糖 25.61g/L、酵母提取物粉（YEP）26.86g/L 和核苷添加物 0.48g/L。最佳发酵条件为：发酵时间 10h、初始 pH 值 6.5、发酵温度 33°C、接种量 1.0%。在此条件下，植物乳杆菌 FS4722 的核苷降解能力提高了 41.9 倍，增强了其在降低尿酸和开发抗痛风食品、保健品和药物方面的潜在应用。
